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Isoform-Specific Membrane Translocation of Protein Kinase C After Ischemic Preconditioning

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Abstract

Mild cerebral anoxic/ischemic/stress insults promote ‘tolerance’ and thereby protect the brain from subsequent ‘lethal’ anoxic/ischemic insults. We examined whether specific activation of PKC α, δ, ∈, or ζ isoforms is associated with ischemic preconditioning (IPC) in rat brain. IPC was produced by a 2-minute global cerebral ischemia. Membrane and cytosolic fractions of the hippocampi were immunoblotted using specific antibodies for PKCα, δ, ∈, and ζ. PKCα showed a significant translocation to the membrane fraction from 30 min to 4 h and PKCδ at 4 h following IPC. In contrast, the membrane/cytosol ratio of PKC∈ showed a tendency to decrease at 30 min and 8 h, and the membrane/cytosol ratio of PKCζ was significantly decreased from 30 min to 24 h following IPC. These findings indicate PKC isoform-specific membrane translocations in the hippocampus after brief global brain ischemia and suggest that activation of PKCα and PKCδ may be associated with IPC-induced tolerance in the rat hippocampus.

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Authors and Affiliations

  1. Department of Neurology, University of Miami School of Medicine, Miami, FL, 33101

    Raul Busto & Miguel A. Pérez-Pinzón

  2. University of Tampere, Kuopio, Finland

    Kaisa Kurkinen, Gundars Goldsteins & Jari Koistinaho

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  1. Kaisa Kurkinen
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  2. Raul Busto
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  5. Miguel A. Pérez-Pinzón
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Kurkinen, K., Busto, R., Goldsteins, G. et al. Isoform-Specific Membrane Translocation of Protein Kinase C After Ischemic Preconditioning. Neurochem Res 26, 1139–1144 (2001). https://doi.org/10.1023/A:1012322906824

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