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Metabolism of NAD+ in Nuclei of Saccharomyces cerevisiae during Stimulation of Its Biosynthesis by Nicotinamide

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Abstract

The activities of nuclear enzymes involved in NAD+ metabolism in Saccharomyces cerevisiae strain 913a-1 and its mutant 110 previously selected as an NAD+ producer were investigated. The presence of extracellular nicotinamide increased the total NAD+ pool in the cells and increased [3H]nicotinic acid incorporation; however, NAD+ concentration in isolated nuclei decreased slightly. The stimulating effect of nicotinamide on intracellular synthesis of NAD+ correlated with increases in ADP-ribosyl transferase, NAD+-pyrophosphorylase, and NAD+ ase activities.

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Authors and Affiliations

  1. Uzbekistan Academy of Sciences, Institute of Microbiology, ul. Abdully Kodiry 7B, Tashkent, 700128, Uzbekistan

    T. G. Gulyamova, D. M. Ruzieva, S. M. Nasmetova, M. R. Shakirzyanova & A. M. Kerbalaeva

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  1. T. G. Gulyamova
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  2. D. M. Ruzieva
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  3. S. M. Nasmetova
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  4. M. R. Shakirzyanova
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  5. A. M. Kerbalaeva
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Gulyamova, T.G., Ruzieva, D.M., Nasmetova, S.M. et al. Metabolism of NAD+ in Nuclei of Saccharomyces cerevisiae during Stimulation of Its Biosynthesis by Nicotinamide. Biochemistry (Moscow) 66, 979–981 (2001). https://doi.org/10.1023/A:1012313408062

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  • DOI: https://doi.org/10.1023/A:1012313408062

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