Abstract
The structure of a “noncanonical” nucleolus of vitellogenic oocytes in the sea urchin Paracentrotus lividuswas studied using the inhibitor of transcription actinomycin D. In the control cells, the nucleolus consists of two separated structural subdomains: the dense fibrillar-granular peripheral area and the fibrillar central area. The nucleolus did not contain subdomains corresponding to the fibrillar center and dense fibrillar component of “typical” nucleoli. After treatment with actinomycin D, numerous argyrophilic granules appeared in the karyoplasm, the intranucleolar DNA became compact, and the nucleolar material was segregated into two or three separated zones, the residual peripheral area being the densest and largest. Lesser zones had a decreased electron density and contained argyrophilic proteins and, apparently, the nucleolar organizer material. These results suggest that, for normal rRNA expression and processing, the presence of structural subdomains in the nucleolus, such as fibrillar complexes and a dense fibrillar component, is not essential.
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REFERENCES
Agard, D.A., Optical Sectioning Microscopy: Cellular Architecture in Three Dimensions, Annu. Rev. Biophys. Bioeng., 1984, vol. 13, pp. 191–219.
Amicura, R.M., Yamada, H., Hirai, S., and Nagana, H., Intracellular Localization of Argyrophilic Proteins in the Maturing Oocyte, Fertilized Egg and Spermatozoa of the Starfish, Gamete Res., 1987, vol. 16, pp. 291–301.
Barbieri, N.R., Izzo, V., Cantons, M., et al., Regulation of Ribosomal RNA Synthesis in Sea Urchin Embryos, Rend. Fis. Ace. Lincei, 1992, vol. 3, pp. 369–374.
de Cárcer, G., and Medina, F.J., Simultaneous Localization of Transcription and Early Processing Markers Allows Dissection of Functional Domains in the Plant Cell Nucleolus, J. Struct. Biol., 1999, vol. 128, pp. 139–151.
Chudinova, E.M., Zatsepina, O.V., Fais, D., et al., Structural and Cytochemical Features of the Nucleoli in Maturing Oocytes of the Sea Urchin Paracentrotus lividus, Tsitologiya, 1996, vol. 38, pp. 1145–1151.
Chudinova, E.M., Zatsepina, O.V., Vorob'ev, I.A., et al., Localization of Argyrophilic Proteins in the Nucleoli of Oocytes of the sea Urchin Paracentrotus lividus, Tsitologiya, 1997, vol. 40, pp. 302–306.
Chudinova, E.M., Zatsepina, O.V., Fais, D., et al., Localization of DNA in the Nucleoli of Oocytes of the Sea Urchin Paracentrotus lividus, Biol. Membrany, 1998, vol. 15, pp. 648–656.
Dundr, M. and Raska, I., Nonisotopic Ultrastructural Mapping of Transcription Sites within the Nucleolus, Exp. Cell Res., 1993, vol. 208, pp. 275–281.
Goessens, G., Nucleolar Structure, Int. Rev. Cytol., 1984, vol. 87, pp. 107–158.
Hernandez-Verdun, D., The Nucleolus Today, J. Cell Sci., 1991, vol. 99, pp. 465–471.
Hozák, P., Cook, P.R., Schofer, C., et al., Site of Transcription of Ribosomal RNA and Intranucleolar Structure in HeLa Cells, J. Cell Biol., 1994, vol. 107, pp. 639–648.
Jordan, E.G., Nucleolar Nomenclature, J. Cell Sci., 1984, vol. 67, pp. 217–220.
Jordan, E.G. and McGovern, J.H., The Quantitative Relationship of the Fibrillar Centres and Other Nucleolar Components to Changes in Growth Conditions, Serum Deprivation and Low Doses of Actinomycin D in Cultured Diploid Human Fibroblasts (Strain MRC-5), J. Cell Sci., 1981, vol. 52, pp. 373–389.
Jordan, E.G., Zatsepina, O.V., and Shaw, P.J., Widely Dispersed DNA within Plant and Animal Nucleoli Visualised by 3-D Fluorescence Microscopy, Chromosoma, 1992, vol. 101, pp. 478–482.
Knibiehler, B., Mirre, C., and Rosset, R., Nucleolar Organizer Structure and Activity in a Nucleolus without Fibrillar Centers. The Nucleolus in an Established Drosophila Cell Line, J. Cell Sci., 1982, vol. 57, pp.351–364.
Knibiehler, B., Mirre, C., Navarro, A., and Rosset, R., Studies on Chromatin Organization in a Nucleolus without Fibrillar Centers, Cell Tissue Res., 1984, vol. 236, pp.279–288.
Lipani, C., Vitturi, R., Sconzo, G., and Barbata, G., Karyotype Analysis of the Sea Urchin Paracentrotus lividus (Echinodermata): Evidence for a Heteromorphic Chromosome Sex Mechanism, Marine Biology, 1996, vol.127, pp. 67–72.
Millonig, G., Bosco, M., and Giambertone, M., Fine Structure in Oogenesis in Sea Urchins, J. Exp. Zool., 1968, vol. 169, pp. 293–314.
Perry, R.P. and Kelley, D.E., Inhibition of RNA Synthesis by Actinomycin D: Characteristic Dose-Response of Different RNA Species, J. Cell Physiol., 1970, vol. 76, pp.127–139.
Puvion-Dutilleul, F., Mazan, S., Nicoloso, M., et al., Alteration of Nucleolar Ultrastructure and Ribosome Biogenesis by Actinomycin D. Implications for U3 snRNP Function, Eur. J. Cell Biol., 1992, vol. 58, pp. 149–162.
Robert-Fortel, I., Junera, H.R., Geraund, G., and Hernandez-Verdun, D., Three-Dimensional Organization of the Ribosomal Genes and Ag-NOR Proteins during Interphase and Mitosis in PtK Cells Studied by Confocal Microscopy, Chromosoma, 1993, vol. 102, pp. 146–157.
Scheer, U. and Benavente, R., Functional and Dynamic Aspects of the Mammalian Nucleolus, BioEssays, 1990, vol. 12, pp. 14–21.
Scheer, U., Xia, B., Merkert, H., and Weisenberger, D., Looking at Christmas Trees in the Nucleolus, Chromosoma, 1997, vol. 105, pp. 470–480.
Shaw, P.J. and Jordan, E.G., The Nucleolus, Annu. Rev. Cell Dev. Biol., 1995, vol. 11, pp. 93–121.
Shaw, P.J., Highett, M.I., Beven, A.F., and Jordan, E.G., The Nucleolar Architecture of Polymerase I Transcription and Processing, EMBO J., 1995, vol. 14, pp. 2896–2906.
Simard, R., Langelier, Y., Mandeville, R., et al., Inhibition as Tool in Elucidating the Structure and Function of the Nucleus, The Cell Nucleus, New York: Academic, 1974, vol. 3, pp. 447–487.
Strouboulis, J. and Wolffe, A.P., Functional Compartmentalization of the Nucleus, J. Cell Sci., 1996, vol. 109, pp.1991–2000.
Verney, C.A. and Moyer, F.H., Fine Structural Changes during Sea Urchin Oogenesis, J. Exp. Zool., 1967, vol. 164, pp. 195–225.
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Sheval', E.V., Gulak, P.V., Kireev, I.I. et al. Characteristics of Actinomycin D-Induced Segregation of a “Noncanonical” Nucleolus of the Sea Urchin Paracentrotus lividus. Russian Journal of Developmental Biology 32, 313–319 (2001). https://doi.org/10.1023/A:1012312718616
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DOI: https://doi.org/10.1023/A:1012312718616