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Identification of a soluble form of the angiopoietin receptor TIE-2 released from endothelial cells and present in human blood

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Abstract

The transmembrane tyrosine kinase TIE-2, the receptor for the angiopoietins-1 and -2, has been shown to be involved in angiogenic processes. Investigating the regulation of TIE-2 expression on endothelial cells, we found that stimulators such as PMA induce a decrease of TIE-2 protein from the cell surface without affecting TIE-2 mRNA. In conditioned media of PMA stimulated endothelial cells, a soluble form of this receptor comprising parts of the extracellular domain can be detected. Using a sandwich ELISA, we were able to detect and quantify TIE-2 receptors in cell lysates (representing the whole transmembrane receptor) and in cell culture supernatants (representing a soluble form of this receptor, sTIE-2). Several factors influencing this shedding process e.g. basic FGF could be identified. Finally, the soluble form of TIE-2 could also be detected in human biological fluids such as sera and plasma from healthy controls.

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Authors and Affiliations

  1. Institute of Molecular Oncology, Tumor Biology Center, Freiburg, Germany

    Petra Reusch, Karin Weindel, Georg Martiny-Baron, Astrid Gödde & Dieter Marmé

  2. Receptor Ligand Technologies GmbH, Braunschweig, Germany Tel:

    Bernhard Barleon

  3. Schering AG, Berlin, Germany

    Gerhard Siemeister

Authors
  1. Petra Reusch
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  2. Bernhard Barleon
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  3. Karin Weindel
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  4. Georg Martiny-Baron
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  5. Astrid Gödde
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  6. Gerhard Siemeister
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  7. Dieter Marmé
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Reusch, P., Barleon, B., Weindel, K. et al. Identification of a soluble form of the angiopoietin receptor TIE-2 released from endothelial cells and present in human blood. Angiogenesis 4, 123–131 (2001). https://doi.org/10.1023/A:1012226627813

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