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Cometabolic biosynthesis of copolyesters consisting of 3-hydroxyvalerate and medium-chain-length 3-hydroxyalkanoates by Pseudomonas sp. DSY-82

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Abstract

A newly isolated strain, designated as Pseudomonas sp. DSY-82, synthesized medium-chain-length polyhydroxyalkanoate (MCL-PHA) copolyesters when grown on alkanoates from hexanoate to undecanoate as the sole carbon source. When used alone, butyrate and valerate supported the growth of the isolate but not PHA production. However, unusual polyesters containing 3-hydroxyvalerate, as well as various MCL 3-hydroxyalkanoate monomeric units, were synthesized when valerate was cofed with either nonanoate or 10-undecenoate, suggesting the formation of monomer units from both substrates. Concentrations of 3-hydroxyvalerate, 3-hydroxyoctanoate, and 3-hydroxydecanoate in the PHAs produced were significantly elevated by the addition of valerate, indicating that the incorporation of these monomer units to PHA occurred primarily through cometabolism. The total amount of these monomer units in the PHAs reached up to 30%. The PHAs produced in this study were most likely random copolyesters as determined by differential scanning calorimetric analysis. This is the first case of microbial synthesis of copolyesters consisting of 3-hydroxyvalerate and MCL 3-hydroxyalkanoate monomer units through cometabolism.

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Authors and Affiliations

  1. Department of Microbiology, Chungnam National University, Daejeon, 305-764, Korea

    Hye Ok Kang, Chung Wook Chung, Hyung Woo Kim & Young Ha Rhee

  2. Department of Polymer Materials, PaiChai University, Daejeon, 302-735, Korea

    Young Baek Kim

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  1. Hye Ok Kang
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  2. Chung Wook Chung
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  3. Hyung Woo Kim
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  4. Young Baek Kim
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  5. Young Ha Rhee
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Correspondence to Young Ha Rhee.

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Kang, H.O., Chung, C.W., Kim, H.W. et al. Cometabolic biosynthesis of copolyesters consisting of 3-hydroxyvalerate and medium-chain-length 3-hydroxyalkanoates by Pseudomonas sp. DSY-82. Antonie Van Leeuwenhoek 80, 185–191 (2001). https://doi.org/10.1023/A:1012214029825

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