Abstract
Wild killer yeasts have been identified as inhibitory to strains used as starters in the production of alcoholic beverages such as beer and wine; therefore, killer or killer-resistant strains have been sought for use in alcoholic fermentations. In the current paper a total of 16 strains belonging to six species were isolated. From two samples of Agave sap (aguamiel) the following yeast strains were isolated: Candida lusitaneae (1), Kluyveromyces marxianus var. bulgaricus (2), and Saccharomyces cerevisiae (capensis) (1). Additionally, in seven samples of pulque (the fermented product), the species C. valida (six strains), S. cerevisiae (chevalieri) (4), S. cerevisiae (capensis) (1), and K. marxianus var. lactis (1) were found. The killer strains were C. valida and K. marxianus var. lactis from pulque and K. marxianus var. bulgaricus from aguamiel. One strain of S. cerevisiae (chevalieri) isolated from pulque which did not show killer activity was, on the other hand, resistant to other killer strains and it had a remarkable ethanol tolerance, suggesting that this strain could be used for alcohol production.
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References
Bonilla-Salinas, M., Lappe, P., Ulloa, M., García-Garibay, M. & Gómez-Ruiz, L. 1995 Isolation and identification of killer yeasts from sugar cane molasses. Letters in Applied Microbiology 21, 115–116.
Carrau, F.M., Neirotti, E. & Gioia, O. 1993 Stuck wine fermentations: effect of killer/sensitive yeast interactions. Journal of Fermentation and Bioengineering 76, 67–69.
Farris, G.A., Mannazzu, I. & Budroni, M. 1991 Identification of killer factor in the yeast genus Metschnikowia. Biotechnology Letters 13, 297–298.
García-Garibay, M. & López-Munguía, A. 1993 Bebidas Alcohólicas no Destiladas. In Biotecnología Alimentaria, eds. García-Garibay, M., Quintero, R. & López-Munguía, A. Ch. 8, pp. 263–311. Mexico City: Limusa ISBN 968–18–4522–6.
Kitamoto, H.K., Ohmono, S. & Nakahara, T. 1993 Selection of killer yeasts (Kluyveromyces lactis) to prevent aerobic deterioration in silage making. Journal of Dairy Science 76, 803–811.
Kono, I. & Himeno, K. 1992 Kluyveromyces yeast having killer activity against Zygosaccharomyces rouxii. Nippon Shokuhin Kogyo Gakkaishi 39, 1135–1139.
Kreger-van Rij, N.J.W. 1984 The Yeasts. A Taxonomic Study. Amsterdam: Elsevier. ISBN 0–444–80421–8.
Lappe, P. & Ulloa, M. 1993 Microbiología del Pulque. In Alimentos Fermentados Indígenas de México, eds. Wacher, M.C. & Lappe, P. pp. 75–79. Mexico City: UNAM. ISBN 968–36–3248–3.
Llorente, P., Marquina, D., Santos, A., Peinado, J.M. & Spencer-Martins, I. 1997 Effect of salt on the killer phenotype of yeasts from olive brines. Applied and Environmental Microbiology 63, 1165–1167.
Medina, K., Carrau, F.M., Gioia, O. & Bracesco, N. 1997 Nitrogen availability of grape juice limits killer yeast growth and fermentation activity during mixed-culture fermentation with sensitive commercial yeast strains. Applied and Environmental Microbiology 63, 2821–2825.
Musmanno, R.A., Di Maggio, T. & Coratza, G. 1999 Studies on strong and weak killer phenotypes of wine yeasts: production, activity of toxin in must, and its effect in mixed culture fermentation. Journal of Applied Microbiology 87, 932–938.
Painting, K.A. & Jackman, P.J.H. 1990 National Collection of Yeast Cultures. Catalogue of Cultures. Agricultural and Food Research Council, Norwich U.K. ISBN 0–7084–0497–9.
Rosini, G. 1989 Killer yeasts: notes on properties and technical use of the character. In Biotechnology Applications in Beverage Production eds. Cantarelli, C. & Lanzarini, G. Ch. 4, pp. 41–48. London: Elsevier Applied Science. ISBN 1–85166–328–2.
Steinkraus, K.H. 1996 Handbook of Indigenous Fermented Foods, 2nd. Edition. pp. 389–397. New York: Marcel Dekker. ISBN 0–8247–9352–8.
Stewart, G.G. & Russell, I. 1985 Tradition meets innovation in brewing. Biotechnology 3, 791–798.
Van Vuuren, H.J.J. & Jacobs, C.J. 1992 Killer yeasts in the wine industry: a review. American Journal of Enology and Viticulture 43, 119–128.
Young, T.W. 1987 Killer Yeasts. In The Yeasts, eds. Rose, A.H. & Harrison, J.S. 2nd. Edition, vol. 2, Ch. 6, pp. 131–164. London: Academic Press. ISBN 0–12–596412–9.
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Estrada-Godina, A., Cruz-Guerrero, A., Lappe, P. et al. Isolation and identification of killer yeasts from Agave sap (aguamiel) and pulque. World Journal of Microbiology and Biotechnology 17, 557–560 (2001). https://doi.org/10.1023/A:1012210106203
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DOI: https://doi.org/10.1023/A:1012210106203