Abstract
Purpose. The intestinal epithelium is considered to be a feasible target for somatic gene therapy. To this end, Caco-2 cells derived from human colon carcinoma were transfected with a mouse interferon-β (IFN-β) expression vector and several stable sublines were established; this hetero-specific cytokine allows unexpected cellular effects to be avoided. Using the highest mouse IFN-β-producing sublines, the mode of IFN secretion was examined.
Methods. The secretion polarity of mouse IFN-β in its gene-transduced Caco-2 sublines was studied in a bicameral culture system in which the chambers were separated by microporous filters.
Results. Mouse IFN-β was secreted to the same extent from both apical and basolateral surfaces of the transduced cells regardless of cell aging.
Conclusions. These results suggest that in the intestinal epithelium exogenous gene products such as IFNs can be delivered to both the luminal and blood sides in vivo. Thus, the intestinal epithelium may be suitable for systemic and local delivery of therapeutic proteins by gene transfer.
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Kawabata, K., Kondo, M., Watanabe, Y. et al. Non-Polarized Secretion of Mouse Interferon-β from Gene-Transferred Human Intestinal Caco-2 Cells. Pharm Res 14, 483–485 (1997). https://doi.org/10.1023/A:1012151616910
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DOI: https://doi.org/10.1023/A:1012151616910