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Taurine Transport in Cultured Choroid Plexus

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Abstract

Purpose. Taurine, a β-amino acid, is a neuromodulator which interacts functionally with the glycinergic, GABAergic, cholinergic and adrenergic systems. Although a continuous cell culture model is not available for the choroid plexus epithelia, we recently described a primary cell culture of rabbit choroid plexus epithelia. The goal of the current study was to determine the suitability of this primary cell culture for the study of the Na+-taurine transporter in the rabbit choroid plexus.

Methods. A primary cell culture of rabbit choroid plexus epithelial cells was grown on semi-permeable filters and kinetics of 3H-taurine uptake were ascertained.

Results. Taurine transport in the cultured choroid plexus cells was Na+-dependent and saturable (Km = 156 μM). The β-amino acids, β-alanine and taurine, significantly inhibited Na+-driven taurine transport whereas L-alanine partially inhibited taurine transport in the cultured cells. In addition, we observed that the activity of the Na+-taurine transporter is affected by exposure to taurine in the media.

Conclusions. These results demonstrate that a Na+-taurine transporter with characteristics similar to those in the intact tissue is expressed in cultured choroid plexus epithelial cells. The transporter may undergo adaptive regulation and play a role in taurine homeostasis in the central nervous system.

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Authors and Affiliations

  1. Graduate Program in Pharmaceutical Chemistry, S-926, University of California, Box 0446, School of Pharmacy, San Francisco, California, 94143

    Vikram K. Ramanathan & Kathleen M. Giacomini

  2. College of Pharmacy, Seoul National University, Seoul, 151-742, Korea

    Suk-Jae Chung

  3. Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, S-926, University of California, Box 0446, School of Pharmacy, San Francisco, California, 94143

    Kathleen M. Giacomini

  4. Department of Anesthesia, University of California, Box 0624, School of Medicine, San Francisco, California, 94143

    Claire M. Brett

Authors
  1. Vikram K. Ramanathan
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  2. Suk-Jae Chung
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  3. Kathleen M. Giacomini
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  4. Claire M. Brett
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Correspondence to Kathleen M. Giacomini.

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Ramanathan, V.K., Chung, SJ., Giacomini, K.M. et al. Taurine Transport in Cultured Choroid Plexus. Pharm Res 14, 406–409 (1997). https://doi.org/10.1023/A:1012074827388

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