Abstract
The variation for α-amylaseisozymes was analyzed in 71 tetraploid wheat (AABB genome)landraces from Ethiopia, including accessions of Triticumdicoccon Shrank, T.turgidum L., T.durum Desf., T.pyramidale Percival and T.aethiopicum Jakubz., by thin-layerpolyacrylamide gel isoelectric focusing. Four zymogram phenotypeswere obtained from all the materials studied. Except inT. dicoccon, however, thestandard pattern of the durum wheat variety, LD222, was predominant.T. dicoccon showed twozymogram types that differed for the absence or consistently weakactivity of band 18(α-Amy-B1)of the malt type. Band 1(α-Amy-B3)of the malt type was fixed in T.dicoccon and present in only 10% of thefree-threshing (FT) types. Generally, the resultsindicated that the variation forα-amylase isozymes in cultivatedtetraploid wheats, including landraces from secondary centers, is lowpossibly due to the founder effect or as a result of selection. Thedata were useful for inferences about the evolutionary history ofEthiopian wheat landraces. It is speculated thatT. dicoccon was the firstwheat to arrive in the Ethiopian highlands ca.5000 years ago. However, it is not known whether the present day FTEthiopian tetraploid wheat landraces are direct descendants ofT. dicoccon, or whetherthey were introduced independently. The clear differences inα-amylase zymogram patterns favor the latterhypothesis. However, the presence of band1 in some of the FT types indicated the occurrence of geneflow between the FT types and T.dicoccon, which may also explain thequantitative nature of spike threshability in these landraces.Possible implications for durum wheat breeding arediscussed.
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References
Ainsworth C.C., Doherty P., Edwards K.G.K., Martienssen R.A. and Gale M.D. 1985. Allelic variation at α-amylase loci in hexaploid wheat. Theor. Appl. Genet. 70: 400–406.
Ban T., Gifu UniversityJapan 1987. Genetic variation and mapping of α-amylase isozymes in hexa-and tetraploid wheats, MSc. 127 p.
Belay G., Merker A. and Tesemma T. 1994. Cytogenetic studies in Ethiopian landraces of tetraploid wheat (Triticum turgidum L.). I. Spike morphology vs. ploidy level and karyomorphology. Hereditas 121: 45–52.
Belay G., Tesemma T., Becker H.C. and Merker A. 1993.Variation and interrelationships of agronomic traits in Ethiopian tetraploid wheat landraces. Euphytica 71: 181–188.
Damania A.B., Hakim S. and Moualla Y. 1992. Evaluation of variation in Triticum dicoccum for wheat improvement in stress environments. Hereditas 116: 163–166.
Feldman M. 1976. Wheats. In: Simmonds N.W (ed.), Evolution of Crop Plants. Longman Group Ltd., London, pp. 120–126.
Habte-Mariam G. and Damessa D. 1994. Characterization and preliminary evaluation of Ethiopian tetraploid wheat germplasm. PGRC/E Germplasm Newsletter 2: 6–10.
Harlan J.R. 1971. Agricultural origins: centers and noncenters. Science 174: 468–473.
Harlan J.R. 1969. Ethiopia: a center of diversity. Econ. Bot. 23: 309–314.
Hart G. 1987. Genetic and biochemical studies of enzymes. In: Heyne E.G (ed.), Wheat and Wheat Improvement. ASA, CSSA, SSSA, Madison, Wisconsin, pp. 199–214.
Helbaek H. 1959. Domestication of food plants in the Old world. Science 130: 365–372.
Ishihara H., Furuta Y., Nishikawa K. and Watanabe N. 1993. Comparative phylogenetic study in amylase isozymes in Gramineae: Why are these enzymes nice marker for phylo-genetical approach in common wheat?. Wheat Inf. Serv. 76: 73–76.
Kochumadhavan M., Tomar S.M.S., Nambisan P.N.N. and Ramanujam S. 1984. Hybrid necrosis and hybrid chlorosis in Indian varieties ofTriticum dicoccum Schubl. Euphytica 33: 853–858.
Morris R. and Sears E.R. 1967. The cytogenetics of wheat and its relatives. In: Quisenberry K.S. and Reitz L.P. (eds), Wheat and Wheat Improvement. ASA, Madison, Wisconsin, pp. 19–87.
Nesbit M. and Samuel D. 1995. From staple crop to extinction? The archaeology and history of the hulled wheats. In: Padulosi S., Hammer K. and Heller J. (eds), Hulled Wheats. Promoting the Conservation and Use of Underutilized and Neglected Crops. 4. Proc. 1st Int. Workshop on Hulled Wheats, 21-22 July Castelvecchio Pascoli, Tuscany, Italy, IPGRI, Rome., pp. 41–100.
Nevo E., Nishikawa K., Furuta Y., Gonokami Y. and Beiles A. 1993. Genetic polymorphisms of α-and β-amylase isozymes in wild emmer wheat, Triticum dicoccoides, in Israel. Theor. Appl. Genet. 85: 1029–1042.
Nishikawa K. 1967. Identification and distribution of necrosis and chlorosis genes in tetraploid wheat. Seiken Ziho 19: 37–42.
Nishikawa K., Ban T. and Furuta Y. 1993. Telocentric mapping of α-amylase loci in wheat. Wheat Inf. Serv. 77: 39–45.
Nishikawa K., Furuta Y., Yamada T. and Kudo S. 1992. Genetic studies of α-amylase isozymes in wheat.VII.Variation in diploid ancestral species and phylogeny of tetraploid wheat. Jpn. J. Genet. 67: 1–15.
Nishikawa K., Furuta Y. and Kudo S. 1988. Genetic studies of α-amylase isozymes in wheat.VI.Variation and differentiation in tetraploid wheat. Jpn. J. Genet. 63: 425–434.
Nishikawa K., Furuta Y. and Wada T. 1980. Genetic studies of α-amylase isozymes in wheat. III. Intraspecific variation in Aegilops squarrosa and birthplace of hexaploid wheat. Jpn. J. Genet. 55: 325–336.
Nishikawa K. and Nobuhara M. 1971. Genetic studies of α-amylase isozymes in wheat. I. Location of genes and variation in tetra-and hexaploid wheat. Jpn. J. Genet. 5: 345–353.
Pagnotta M., Tanzarella O.A., Iacono E., De Pace C., Scarasica Mugnozza G.T. and Porceddu E. 1998. Genetic variation for RFLPs in durum wheat germplasm from Ethiopian regions. Proc. 9th Int. Wheat Genet. Symp., Saskatoon, Saskatchewan, Canada. 41-44.
Perrino P., Laghetti G., D'Antuono L.F., Al Ajlouni M., Kanbertry M., Szabo A.T. et al. 1996. In: Padulosi S., Hammer K. and Heller J. (eds), Hulled Wheats. Promoting the conservation and use of underutilized and neglected crops. 4. Proc. 1st Int.Workshop on Hulled Wheats, 21-22 July 1995, Castelvecchio Pascoli, Tuscany, Italy, IPGRI, Rome., pp. 101–119.
Simonetti M.C., Bellomo M.P., Laghetti G., Perrino P., Simeone R. and Blanco A. 1999. Quantitative trait loci influencing free-threshing habit in tetraploid wheats. Genet. Res. Crop Evol. 46: 267–271.
Tesfaye T., Getachew B. and Worede M. 1991. Morphological diversity in tetraploid wheat landrace populations from the central highlands of Ethiopia. Hereditas 114: 171–176.
Tsegaye S. 1996. Estimation of outcrossing rate in landraces of tetraploid wheat (Triticum turgidum L.). Plant Breed. 115: 195–197.
Tsegaye S., Tesemma T. and Belay G. 1996. Relationships among tetraploid wheat (Triticum turgidum L.) landrace populations revealed by isozyme markers and agronomic traits. Theor. Appl. Genet. 93: 600–605.
Tsunewaki K. and Nakai Y. 1973. Consideration on the origin and speciation of four groups of wheat from the distribution of necrosis and chlorosis genes. Proc. 4th Int. Wheat Genet. Symp., Columbia, Missouri. 123–129.
Vavilov N.I. 1951. The Origin,Variation, Immunity and Breeding of Cultivated Plants. Chronica Bot. 13: 1–351.
Zeven A.C. 1998. Landraces: A review of definitions and classifications. Euphytica 104: 127–139.
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Belay, G., Furuta, Y. Zymogram patterns of α-amylase isozymes inEthiopian tetraploid wheat landraces: insight into their evolutionaryhistory and evidence for gene flow. Genetic Resources and Crop Evolution 48, 507–512 (2001). https://doi.org/10.1023/A:1012055915502
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DOI: https://doi.org/10.1023/A:1012055915502