Abstract
The Wx-D1 protein (granule-bound starch synthase) of Kanto 107, Tanikei A6099 (low amylose line), and Tanikei A6599-4 (waxy line) has been analyzed by SDS-PAGE, peptide mapping and DNA sequencing. Kanto 107 and Tanikei A6099 have the same amino acid sequences in the mature protein, but amino acid substitution (alanine to threonine) occurs at position 258 in the mature protein in Tanikei A6599-4. A comparison of deduced amino acid sequences of the mature Wx-D1 protein in these lines indicates that point mutation in the Wx-D1 gene of Tanikei A6599-4 is responsible for its waxy character.This mutant waxy wheat does not show a reduction in amylose content identicalto other waxy wheats, which probably reduces activity of the GBSS I enzyme but does not to a complete loss of activity. We discuss the function of the mutant Wx-D1 protein in starch synthesis.
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References
Ainsworth, C., J. Clark & J. Balsdon, 1993. Expression, organization and structure of the genesencoding the waxy protein (granule-bound starch synthase) in wheat. Plant Mol Biol 22: 67–72.
Clark, J.R.,M. Robertson & C.C. Ainsworth, 1991. Nucleotide sequence of a wheat (Triticum aestivum L.) cDNA clone encoding the waxy protein. Plant Mol Biol 16: 1099–1101.
Echt, C.S. & D. Schwartz, 1981. Evidence for theinclusion of controlling elements within the structural gene at the waxy locus in maize. Genetics 99: 275–284.
Fujita, N., A. Wadano, S. Kozaki, K. Takaoka, S. Okabe & T. Taira, 1996. Comparison of the primary structureof Waxy proteins (granule-bound starch synthase) between polyploid wheats and related diploid species. Biochem Genet 34: 403–413.
Flipse, E., C.J.A.M. Keetels, E. Jacobson & R.G.F. Visser, 1996. The dosage effect of the wildtypeGBSS allele is linear for GBSS activity but not for amylose content: absence of amylose has distinct influence on the physico-chemical properties of starch. Theor Appl Genet 92: 121–127.
Hirano, H. & Y. Sano, 1991. Molecularcharacterization of the waxy locus of Rice (Oryza sativa). Plant Cell Physiol 32: 989–997.
Hsieh, J., C. Liu & Y.C. Hsing, 1996. Molecular cloning of a sorghum cDNA encoding the seed waxy protein. Plant Physiol 112: 1275.
Kiribuchi-Otobe, C., T. Yanagisawa & H. Yoshida, 1998. Wheat mutant with waxy starch showing stablehot paste viscosity. Cereal Chem 75: 671–672.
Kiribuchi-Otobe, C., T. Nagamine, T. Yanagisawa, M. Ohnishi & I. Yamaguchi, 1997. Production of hexaploid wheats with waxy endosperm character. Cereal Chem 74: 72–74.
Klösgen, R.B., A. Gierl, Z. Schwarz-Sommer & H. Saedler, 1986. Molecular analysis of the waxy locus of Zeamays. Mol Gen Genet 203: 237–244.
Murai, J., T. Taira & D. Ohta, 1999. Isolation andcharacterization of the three Waxy genes encoding the granule-bound starch synthase in hexaploid wheat. Gene 234: 71–79.
Nakamura, T., M. Yamamori, H. Hirano & S. Hidaka, 1993. Decrease of waxy (Wx) protein in two commonwheat cultivars with low amylose content. Plant Breed 111: 99–105.
Nakamura, T., M. Yamamori, H. Hirano, S. Hidaka & T. Nagamine, 1995. Production of waxy (amylose-free) wheats. Mol Gen Genet 248: 253–259.
Oda, M., Y. Yasuda, S. Okazaki, Y. Yamaguchi & Y. Yokoyama, 1980. A method of flour quality assessment forJapanese noodles. Cereal Chem 57: 253–254.
Oda, S., C. Kiribuchi & H. Seko, 1992. A bread wheatmutant with low amylose content induced by ethyl methanesulphonate. Japan J Breed 42: 151–154.
Rohde, W.,D. Becker & F. Salamini, 1988. Structural analysis of the waxy locus from Hordeum vulgare. Nucl Acid Res 16: 7185–7186.
Yamaguchi, I., C. Kiribuchi-Otobe, T. Yanagisawa, T. Ushiyama, H. Seko, Y. Amano, S. Miyagawa, A. Kuroda & S. Oda, 1998. Breeding of wheat Norin-PL 7 (Tanikei A6099) New germplasm accession with low amylose content. Bull Natl Agric Res Cent 29: 1–11.
Yamamori, M., T. Nakamura & A. Kuroda,1992. Variations in the content of starch-granule bound protein among several Japanese cultivars of common wheat (Triticum aestivum L.). Euphytica 64: 215–219.
Yamamori, M., T. Nakamura, R. Endo & T. Nagamine 1994.Waxy protein deficiency and chromosomal location of coding genes in common wheat. Theor Appl Genet 89: 179–184.
Yasui, T., T. Sasaki, J. Matsuki & M. Yamamori, 1997. Waxy endosperm mutants of bread wheat (Triticumaestivum L.) and their starch properties. Breed Sci 47: 161–163.
Yasui, T., T. Sasaki & J. Matsuki, 1998.Waxy bread wheat mutants, K107Wx1 and K107Wx2, have a new allele on Wx-D1 locus. Breed Sci 48: 405–407.
Vrinten, P., T. Nakamura & M. Yamamori, 1999. Molecular characterization of waxy mutations in wheat. MolGen Genet 261: 463–471.
Wang, Z., Z. Wu, Y. Xing, F. Zheng, X. Guo, W. Zhang & M. Hong, 1990.Nucleotide sequence of rice waxy gene. Nucl Acids Res 18: 5898.
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Yanagisawa, T., Kiribuchi-Otobe, C. & Yoshida, H. An alanine to threonine change in the Wx-D1 protein reduces GBSS I activity in waxy mutant wheat. Euphytica 121, 209–214 (2001). https://doi.org/10.1023/A:1012051705517
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DOI: https://doi.org/10.1023/A:1012051705517