Abstract
Intergeneric hybrids were obtained between Sandersonia aurantiaca and Littonia modesta using ovule culture. The embryos were rescued by culturing 14 to 30 day old ovules. The ovules were cultured on modified KM medium for varying lengths of time until they germinated. After germination the embryo-derived-plantlets were transferred to modified growth regulator-free MS medium on which they developed tubers and became quiescent. The quiescent tubers could be successfully transferred to soil. The hybrid nature of both the putative Sandersonia × Littonia and the Littonia × Sandersonia hybrids was indicated by flow cytometry that showed the hybrid plants had a DNA content midway between that of the two parents. Mitotic and meiotic chromosome counts from S. aurantiaca, L. modesta and the hybrids gave chromosome numbers of (2n=) 24, 22 and 23 respectively. Morphological analyses of the leaves and flowers showed that the hybrids displayed features that were intermediate between both parents. Hybrids were male and female sterile. No morphological differences were observed between the two hybrids.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alexander, M.P., 1969. Differential staining of aborted and nonaborted pollen. Stain Tech44(3): 117–122.
Arisumi, T., 1985. Rescuing abortive Impatiens hybrids through aseptic culture of ovules. JAm Soc Hort Sci 110(2): 273–276.
Clark, G.E., 1995. Effects of storage temperature and duration on thedormancy of Sandersonia aurantiaca tubers. NZ J Crop Hort Sci 23(4): 455–460.
Dickson, E.E.,K. Arumuganathan, S. Kresovich & J.J. Doyle, 1992. Nuclear DNA content variation within the Rosaceae. Am J Bot 79: 1081–1086.
Finch, R.A., J.B. Smith & M.D. Bennett, 1981. Hordeum and Secale mitoticchromosomes lie apart in a hybrid. J Cell Sci 52: 391–403.
Finnie, J.F. & J. van Staden, 1989. In vitro propagation of Sandersonia and Gloriosa. Plant Cell, Tissue and Organ Culture 19: 151–158.
Galbraith, D.W.,K.R. Harkins, J.M. Maddox, N.M. Ayres, D.P. Sharma & E. Firoozabady, 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049–1051.
Gamborg, O.L., R.A. Miller & K. Ojima,1968. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158.
Grant, J.E., 1989. Soybean: wide hybridization through embryo culture. In: Y.P.S. Bajaj (Ed.), Biotechnology inAgriculture and Forestry, Vol 10, Legumes and Oil Seed Crops, pp. 134–148. Springer-Verlag, Heidelberg.
Grant, J.E., A.H.D. Brown & J.P. Grace, 1984a. Cytological and isozyme diversity in Glycine tomentella Hayata(Leguminosae). Aust J Bot 32: 665–677.
Grant, J.E., J.P. Grace, A.H.D. Brown & E. Putievsky, 1984b.Interspecific hybridization in Glycine Willd. Subgenus Glycine (Leguminosae). Aust J Bot 32: 655–663.
Heslop-Harrison, J.S., 1990. Gene expression and parental dominance in hybrid plants. Development (Suppl.): 21–28.
Heslop-Harrison, J.S. & M.D. Bennett, 1984. Chromosome orderpossible implications for development. J Emb & Exp Morph 83 (Suppl.): 51–73.
Heslop-Harrison, J.S. & M.D. Bennett, 1990. Nuclear architecture inplants. Trends in Genet 6: 401–405.
Hopping, M.E., 1993. Preparation and preservation of nuclei from planttissues for quantitative DNA analysis by flow cytometry. NZ J Bot 31: 391–401.
Kao, K.N. & M.R. Michayluk, 1975. Nutritional requirements for growth of Vicia hastajana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110.
Kho, Y.O. & J. Baer, 1968. Observing pollentubes by means of fluorescence. Euphytica 17: 298–302.
Kishi, F., Y. Kagami, M. Shinohara, S Hatano & H. Tsurushima, 1994. Production of an interspecific hybrid in Gypsophila by ovule-embryo culture. Euphytica 74: 85–90.
Morgan, E.R., G.K. Burge, J.F. Seelye, J.E. Grant & M.E. Hopping, 1995. Interspecific hybridisationbetween Limonium perigrinum Bergius and Limonium purpuratum L. Euphytica 83: 215–224.
Morgan, E.R.,G.K. Burge, J.F. Seelye, J.E. Grant & M.E. Hopping, 1998. Production of interspecific hybrids between Limonium perezii (Stapt) Hubb. and Limonium sinuatum (L.) Mill. Euphytica 102: 109–115.
Murashige, T. & F. Skoog,1962. A revised medium for rapid growth and bioassays with tobacco tissues cultures. Physiol Plant 15: 473–479.
Neves, N., A. Castilho, M. Silva, J.S. Heslop-Harrison & W. Viegas, 1997. Genomic interactions: geneexpression, DNA methylation and nuclear architecture. Chromosomes Today 12: 182–200.
Van Tuyl, J.M.,K. Van-Sande, R. Van-Dien & D. Straathof, 1990. Overcoming interspecific crossing barriers in Lilium by ovary and embryo culture. Acta Hortic 266: 317–322.
Van Tuyl, J.M., M.P. Van-Dien, M.G.M. Van-Creij, T.C.M. Van-Kleinwee, J. Franken & R.J. Bino, 1991. Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses. Plant Sci 74: 115–126.
Van Tuyl, J.M. & E. Boon, 1997. Variation in nuclear DNA content in the genus Lilium. Acta Hort 430: 829–835.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Morgan, E., Burge, G., Seelye, J. et al. Wide crosses in the Colchicaceae: Sandersonia aurantiaca (Hook.) × Littonia modesta (Hook.). Euphytica 121, 343–348 (2001). https://doi.org/10.1023/A:1012034015686
Issue Date:
DOI: https://doi.org/10.1023/A:1012034015686