Abstract
Sequence analysis of several Bacillus subtilismutants with increased activity of flavokinase/FAD-synthase and the results of Nothern hybridization showed that the TTGCCG-17 n -TACATT motif localized to the C-end of the truBgene is a regulatory region that controls the ribCgene at the level of transcription.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Gusarov, I.I., Kreneva, R.A., Iomantas, Yu.A., et al., The Primary Structure and Functional Activity of the Bacillus subtilis ribC Gene, Mol. Biol. (Moscow), 1997, vol. 31, no. 5, pp. 825-830.
Mack, M., van Loon, A.P.G.M., and Hohman, H.P., Regulation of Riboflavin Biosynthesis in Bacillus subtilis Is Affected by the Activity of the Flavokinase/Flavin Adenine Dinucleotide Synthetase Encoded by ribC, J. Bacteriol., 1998, vol. 180, pp. 950-955.
Kreneva, R.A., Polanuer, B.M., Solov'eva, I.M., and Perumov, D.A., Analysis of the Operatorlike Structure That Regulates the Activity of the Bacillus subtilis ribC Gene, Genetika (Moscow), 1999, vol. 35, no. 3, pp. 409-411.
Bresler, S.E., Glazunov, E.A., Chernik, T.P., et al., Study of the Riboflavin Biosynthesis Operon in Bacillus subtilis: V. Flavin Mononucleotide and Flavin Adenine Dinucleotide as Effectors of the Riboflavin Operon, Genetika (Moscow), 1973, vol. 9, no. 3, pp. 84-92.
Perumov, D.A., Glazunov, E.A., and Gorinchuk, G.F., Study of the Riboflavin Biosynthesis Operon in Bacillus subtilis: XVII. Analysis of the Regulatory Function of Intermediate Products and Their Derivatives, Genetika (Moscow), 1986, vol. 22, no. 5, pp. 748-754.
Kreneva, R.A. and Perumov, D.A., Genetic Mapping of Regulatory Mutations of Bacillus subtilis Riboflavin Operon, Mol. Gen. Genet., 1990, vol. 222, pp. 467-469.
Saito, H. and Miura, K.I., Preparation of Transforming DNA by Phenol Treatment, Biochim. Biophys. Acta, 1963, vol. 42, pp. 619-629.
Sanger, F., Nicklen, S., and Coulson, A.R., DNA Sequencing with Chain-Terminating Inhibitors, Proc. Natl. Acad. Sci. USA, 1977, vol. 74, pp. 5463-5467.
Kreneva, R.A. and Perumov, D.A., Riboflavin Biosynthesis in Bacillus subtilis: Study of the Antivitamin Activity of Analogs with Modified Ribityl, Biotechnologiya, 1994, no. 3, pp. 6-10.
Kunst, F., Ogasavara, N., Moszer, I., et al., The Complete Genome Sequence of the Gram-Positive Bacterium Bacillus subtilis, Nature, 1997, vol. 390, pp. 249-256.
Shazand, K., Tucker, J., Grunberg-Monago, M., et al., Similar Organization of the nusA-infB Operon in Bacillus subtilis and Escherichia coli, J. Bacteriol., 1993, vol. 175, no. 10, pp. 2280-2287.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kreneva, P.A., Solov'eva, I.M., Errais, L.L. et al. Investigation of the Regulation Mechanism of theribCGene Activity in Bacillus subtilis. Russian Journal of Genetics 37, 1090–1093 (2001). https://doi.org/10.1023/A:1011978018345
Issue Date:
DOI: https://doi.org/10.1023/A:1011978018345