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Nitrification rates in a saline medium at different dissolved oxygen concentrations

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Abstract

A kinetic study was carried out in a saline medium to assess the effect of O2 on the two-step nitrification process: for the first nitritation step, 2–26 mg dissolved O2 (DO) l−1 was used and for the second nitratation step, 0.5–24 mg DO l−1 was used. Nitritation rate was measured in the presence of sodium azide so as to inhibit nitratation. Ammonia-oxidizing (AOB) and nitrite-oxidizing (NOB) bacterial in the mixed culture were determined by 16 S rRNA hybridization. The affinity constants for oxygen O2 of the AOB and the NOB were 1.66 mg O2 l−1 and 3 mg O2 l−1 respectively. The larger than the previously reported values of these constants might be due to the high salt content in the medium. High O2 concentrations did inhibit the nitrification rate.

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References

  • APHA (1985) Standard Method for the Examination of Water and Wastewater, 16th edn. Washington, DC: American Public Health Association.

    Google Scholar 

  • Aspé E,Martí MC,Roeckel M (1997) Water Res. 31: 2147-2160.

    Google Scholar 

  • Beccari M,Di Pinto AC,Ramadori R,Tomei MC (1992) Water Res. 26: 1099-1104.

    Google Scholar 

  • Chech JS,Chudoba J,Grau P (1984) Water Sci. Technol. 17: 259-272.

    Google Scholar 

  • Drtil M,Németh P,Bodík I (1993) Water Res. 27: 35-39.

    Google Scholar 

  • Garrido JM,van Benthum WAJ,van Loosdrecht MCM,Heijnen JJ (1997) Biotechnol. Bioeng. 53: 168-178.

    Google Scholar 

  • Ginestet P,Audic J,Urbain V,Block J (1998). Appl. Environ. Microbiol. 64: 2266-2268.

    Google Scholar 

  • Grady Jr, JCPL,Smets BF,Barbeau DS (1996) Water Res. 30: 742-748.

    Google Scholar 

  • Hanaki K,Wantawin C,Ohgaki S (1990) Water Res. 24: 297-302.

    Google Scholar 

  • Hovanec T,Taylor L,Blakis A,Delong E (1998) Appl. Environ. Microbiol. 64: 258-264.

    Google Scholar 

  • Hunik JH,Meijer HJG,Tramper J (1992) Appl. Microbiol. Biotechnol. 37: 802-807.

    Google Scholar 

  • Hunik JH,Meijer HJG,Tramper J (1993) Appl. Microbiol. Biotechnol. 40: 442-448.

    Google Scholar 

  • Jayamohan SJ,Ohgaki S,Hanaki K (1988) Water Supply 8: 141-150.

    Google Scholar 

  • Juretschko S,Timmermann G,Schmid M (1998) Appl. Environ. Microbiol. 64: 3042-3051.

    Google Scholar 

  • Laanbroeck HL,Bodelier PLE,Gerards S (1994) Archiv. Microbiol. 161: 156-162.

    Google Scholar 

  • Lazarova V,Nogueira R,Manem J,Melo L (1998) Water Sci. Technol. 37: 189-193.

    Google Scholar 

  • Sánchez O,Aspé E,Roeckel M (2000) In: Anales XIV Congreso Chileno de Ingeniería Química (Proceedings of the XIVth Chilean Meeting of Chemical Engineering), Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Santiago de Chile, Santiago de Chile, 401-405.

    Google Scholar 

  • Schramm A,de Beer D,Wagner M,Amann R (1998) Appl. Environ. Microbiol. 64: 3480-3485.

    Google Scholar 

  • Sharma B,Ahlert RC (1977) Water Res. 11: 897-925.

    Google Scholar 

  • Stenstrom MK,Poduska RA (1980) Water Res. 14: 643-649.

    Google Scholar 

  • Stenstrom MK,Song S (1991) J. Water Pollut. Control Fed. 63: 208-219.

    Google Scholar 

  • Sthal DA (1997) Molec. Ecol. 4: 535-542.

    Google Scholar 

  • Summers WC (1970) Anal. Biochem. 33: 459-463.

    Google Scholar 

  • Vidal G,Aspé E,Martí MC,Roeckel M (1997) Biotechnol. Lett. 19: 117-121.

    Google Scholar 

  • Wiesmann U (1994) Adv. Biochem. Eng. 51: 113-154.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Departamento de Ingeniería Química, Chile

    Omar Sánchez, Estrella Aspé & Marlene Roeckel

  2. Departamento de Farmacología, Universidad de Concepción, Casilla 160-C, Concepción, Chile

    María C. Martí

Authors
  1. Omar Sánchez
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  2. María C. Martí
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  3. Estrella Aspé
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  4. Marlene Roeckel
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Sánchez, O., Martí, M.C., Aspé, E. et al. Nitrification rates in a saline medium at different dissolved oxygen concentrations. Biotechnology Letters 23, 1597–1602 (2001). https://doi.org/10.1023/A:1011977629398

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  • DOI: https://doi.org/10.1023/A:1011977629398

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