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Microbial transformation of 18β-glycyrrhetinic acid by Sphingomonas paucimobilis strain G5

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Abstract

The effects of the oxygenase inhibitors, 1-aminobenzotriazole (ABT), ketoconazole, metyrapone and proadifen, on the metabolism of 18β-glycyrrhetinic acid (18β-GRA) in Sphingomonas paucimobilis strain G5 were investigated. Strain G5 transformed 18β-GRA into a major new metabolite (M-D) in the presence of 1 mM ABT or metyrapone. M-D was purified and identified as 3β-hydroxy-11-oxo-olean-12-en-24,30-dioic acid by NMR and MS. Based on the structure of M-D, we propose the metabolic pathway of 18β-GRA in strain G5.

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Authors and Affiliations

  1. Central Laboratory, Lotte Co., Ltd., 3-1-1 Numakage, Urawa, Saitama, 336-0027, Japan

    Keishiro Yoshida

  2. Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

    Hiroshi Habe, Hisakazu Yamane & Toshio Omori

  3. Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Kazuo Furihata

Authors
  1. Keishiro Yoshida
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  2. Kazuo Furihata
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  3. Hiroshi Habe
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  4. Hisakazu Yamane
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  5. Toshio Omori
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Yoshida, K., Furihata, K., Habe, H. et al. Microbial transformation of 18β-glycyrrhetinic acid by Sphingomonas paucimobilis strain G5. Biotechnology Letters 23, 1619–1624 (2001). https://doi.org/10.1023/A:1011976300594

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  • DOI: https://doi.org/10.1023/A:1011976300594

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