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Mixotrophic culture of Chlorella pyrenoidosa with olive-mill wastewater as the nutrient medium

Abstract

With olive-mill wastewater (`alpechín') as the nutrient medium, theinfluence of specific rate of aeration and initial alpechín concentrationhave been analysed in cultures of Chlorella pyrenoidosa, exposed bothto continuous and intermittent illumination (12/12 h light/dark cycles). The stirring rate in the bioreactor, as well as pH and temperature werefixed previously at 180 rpm, 6.5 and 30 °C, respectively. Themaximum specific growth rate (μm) and biomass productivity(b) were determined as kinetic parameters. The chlorophyll, protein andcarbohydrate contents were evaluated, as well as the fatty-acid compositionof the lipid fraction. The experimental conditions most conducive to abalanced biomass composition with regard to proteins and lipids were: initial alpechín concentration of 10% (v/v), continuous illumination,and aeration rate of 1 L (litre cell suspension)-1 min-1. Under these conditions, the highest values of μm and b wereclose to 0.04 h-1 and 1.4 10-3 g L-1 h-1, respectively.

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Sánchez, S., Martínez, M., Espejo, M. et al. Mixotrophic culture of Chlorella pyrenoidosa with olive-mill wastewater as the nutrient medium. Journal of Applied Phycology 13, 443–449 (2001). https://doi.org/10.1023/A:1011929723586

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  • alpechín
  • biomass productivity
  • Chlorella pyrenoidosa
  • fatty acids
  • growth rate
  • microalga
  • mixotrophic culture
  • protein