Abstract
With olive-mill wastewater (`alpechín') as the nutrient medium, theinfluence of specific rate of aeration and initial alpechín concentrationhave been analysed in cultures of Chlorella pyrenoidosa, exposed bothto continuous and intermittent illumination (12/12 h light/dark cycles). The stirring rate in the bioreactor, as well as pH and temperature werefixed previously at 180 rpm, 6.5 and 30 °C, respectively. Themaximum specific growth rate (μm) and biomass productivity(b) were determined as kinetic parameters. The chlorophyll, protein andcarbohydrate contents were evaluated, as well as the fatty-acid compositionof the lipid fraction. The experimental conditions most conducive to abalanced biomass composition with regard to proteins and lipids were: initial alpechín concentration of 10% (v/v), continuous illumination,and aeration rate of 1 L (litre cell suspension)-1 min-1. Under these conditions, the highest values of μm and b wereclose to 0.04 h-1 and 1.4 10-3 g L-1 h-1, respectively.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Becker EW (1986) Nutritional properties of microalgae: potentials and constraints. In Richmond A (ed.), Handbook of Microalgal Mass Culture, CRC Press, Boca Raton, FL, pp. 339–420.
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Chem. 72: 248–254.
Camacho F, Martínez ME, Sánchez S (1987) Crecimiento heterotrófico de Chlorella pyrenoidosa. Afinidad 411: 395–399.
Camacho F, Martínez ME, Sánchez S (1988) Crecimiento heterotrófico de Chlorella pyrenoidosa sobre monosacáridos. An. Quim. 84: 349–354.
Espinola JB (1994) Estudio del crecimiento mixotrófico de Chlorella pyrenoidosa en un fotobiorreactor discontinuo. PhD thesis, University of Granada, Faculty of Science, Granada, Spain.
Fiestas JA (1977) Depuración de aguas residuales en las industrias de aceitunas y aceites de oliva. Grasas y Aceites 28: 113–121.
Janer L (1980) Contaminación de las aguas por el alpechín y posibles soluciones al problema. Grasas y Aceites 31: 273–279.
Jeffrey SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophylls a, b, c1, and c2 in higher plants, algae and natural phytoplankton. Biochem. Physiol. Pflanzen. 167: 191–194.
Karlander EP, Krauss RW (1966) Responses of heterotrophic cultures of Chlorella vulgaris Beyerinck to darkness and light. I. Pigment and pH changes. Plant Physiol 41: 1–6.
Lepage G, Roy CC (1984) Improved recovery of fatty acid through direct transesterification without prior extraction or purification. J. Lipid Res. 25: 1391–1396.
Martínez ME, Bravo V, Sánchez S, Molina E (1991) Determining the kinetic parameters characteristic of microalgal growth. Chem. Engng Ed. 25: 145–149.
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426–428.
Oh-Hama T, Miyachi S (1988) Chlorella. In Borowitzka MA, Borowitzka LJ (ed.), Micro-algal Biotechnology, Cambridge University Press, Cambridge.
Parsons TR, Maita Y, Lalli CM (1984) A Manual of Chemical and Biological Methods for Seawater Analysis, Pergamon Press, New York, 173 pp.
Sánchez S, Martínez ME, Espejo T, Delgado A (1996) Production of microalgae from olive mill wastewater. Int. Biodet. Biodegr. 38: 245–247.
Sánchez S, Martínez ME, Espinola F (2000) Biomass production and variability of the microalga Isochrysis galbana in relation to culture medium. Biochem. Engng J. 6: 13–18.
Sánchez S, Martínez ME, Molina E, de la Casa JA (1994) Skeletonema costatum as a potential source of fatty acids and single-cell protein (SCP): The effect of pH on growth rate and biomass composition. J. mar. Biotechnol. 2: 23–26.
Vladimirova MG, Rudova TS, Shatilov VR, Salamatova LV, Nazarova GD (1979) Comparative characterization of Chlorella pyrenoidosa Chick 82 and Chlorella pyrenoidosa Pringsheim 82T under intensive culture conditions. Soviet Plant Physiol., Transl. 26: 913–920.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sánchez, S., Martínez, M., Espejo, M. et al. Mixotrophic culture of Chlorella pyrenoidosa with olive-mill wastewater as the nutrient medium. Journal of Applied Phycology 13, 443–449 (2001). https://doi.org/10.1023/A:1011929723586
Issue Date:
DOI: https://doi.org/10.1023/A:1011929723586