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Complementation of CMV Subgroup IA Strains in Replicase-mediated Resistant Tobacco Plants after Co-inoculation with Different Cucumoviruses

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Abstract

Replicase-mediated tobacco plants are highly resistant to the Fny strain of Cucumber mosaic virus (CMV) and closely related subgroup IA strains. Two of these subgroup IA strains, Fny- and M-CMV, were co-inoculated with different resistance breaking cucumoviruses to nontransformed and transformed tobacco plants. RT-PCR analyses of single CMV RNAs were performed to study potential complementation of the subgroup IA strains by the resistance breaking cucumoviruses. After co-inoculation of M-CMV with PII-CMV, RNAs 1, 2 and 3 from M-CMV were detected in systemically infected leaves of control plants, whereas in noninoculated parts of replicase-mediated resistant plants only M-CMV RNAs 1 and 3 were found. Western blot studies confirmed the expression of M-CMV coat protein after co-inoculation with PII-CMV in leaves of transgenic plants. These plants also exhibited M-CMV typical yellow spots. M-CMV/TAV co-inoculated transgenic plants contained only M-CMV RNA 3, but no M-CMV RNAs 1 and 2. No M-CMV typical yellow spots were observed in these plants. Our data suggest different types of complementation of M-CMV in replicase-mediated resistant plants by PII-CMV and TAV in trans potentially leading to new RNA combinations in transformed plants compared to nontransformed plants.

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References

  • Anderson JM, Palukaitis P and Zaitlin M (1992) A defective replicase gene induces resistance to cucumber mosaic virus in transgenic tobacco plants. Proc Natl Acad Sci USA 89: 8759-8763

    Google Scholar 

  • Anonymous (1998) Detection and biodiversity of cucumber mosaic cucumovirus. Conclusions from a ringtest of European Union Cost 823 (New technologies to improve phytodiagnosis). J Plant Pathol 80: 133-149

    Google Scholar 

  • Brigneti G, Voinnet O, Li W-X, Ji L-H, Ding S-W and Baulcombe DC (1998) Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J 17: 6739-6746

    Google Scholar 

  • Carr JP and Zaitlin M (1993) Replicase-mediated resistance. Semin Virol 4: 339-347

    Google Scholar 

  • Carr JP, Gal-On A, Palukaitis P and Zaitlin M (1994) Replicasemediated resistance to cucumber mosaic virus in transgenic plants involves suppression of both virus replication in the inoculated leaves and long-distance movement. Virology 199: 439-447

    Google Scholar 

  • Daniels J and Campbell RN (1992) Characterization of cucumber mosaic virus isolates from california. Plant Dis 76: 1245-1250

    Google Scholar 

  • Ding SW, Li W-X and Symons RH (1995) A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. Embo J 14: 5762-5772

    Google Scholar 

  • Dougherty WG, Lindbo JA, Smith HA, Parks TD, Swaney S and Proebsting WM (1994) RNA-mediated virus resistance in transgenic plants: Exploitation of a cellular pathway possibly involved in RNA degradation. Mol Plant-Microbe Interact 7: 544-552

    Google Scholar 

  • Fraile A, Alonso-Prados JL, Aranda MA, Bernal JJ, Malpica JM and Garcia-Arenal F (1997) Genetic exchange by recombination or reassortment is infrequent in natural populations of a tripartite RNA plant virus. J Virol 71: 934-940

    Google Scholar 

  • Hellwald K-H and Glenewinkel D (1999) A new genotype within cucumber mosaic virus subgroup I: Molecular characterization of the polymerase encoding RNA 2 and its capability to overcome replicase-mediated resistance. J Phytopathol 147: 671-677

    Google Scholar 

  • Hellwald K-H and Palukaitis P (1994) Nucleotide sequence and infectivity of cucumber mosaic cucumovirus (strain K) RNA 2 involved in breakage of replicase-mediated resistance. J Gen Virol 75: 2121-2125

    Google Scholar 

  • Hellwald K-H and Palukaitis P (1995) Viral RNA as a potential target for two independent mechanisms of replicasemediated resistance against cucumber mosaic virus. Cell 83: 937-946

    Google Scholar 

  • Hellwald K-H, Glenewinkel D and Hauber H (2000) Increased symptom severity in pepper plants after co-inoculation with two cucumber mosaic virus subgroup I strains in comparison to single inoculations of the corresponding wildtype strains. Z PflKrankh PflSchutz 107: 368-375

    Google Scholar 

  • Higgins DG and Sharp PM (1988) CLUSTAL: A package for performing multiple sequence alignments on a microcomputer. Gene 73: 237-244

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685

    Google Scholar 

  • Masuta C, Ueda S, Suzuki M and Uyeda I (1998) Evolution of a quadripartite hybrid virus by interspecific exchange and recombination between replicase components of two related tripartite RNA viruses. Proc Natl Acad Sci USA 95: 10487-10492

    Google Scholar 

  • Mossop DW, Francki RIB and Grivell CJ (1976) Comparative studies on tomato aspermy and cucumber mosaic viruses. V. Purification and properties of a cucumber mosaic virus inducing severe chlorosis. Virology 74: 544-546

    Google Scholar 

  • Mueller E, Gilbert J, Davenport G, Brigneti G and Baulcombe DC (1995) Homology dependent resistance: transgenic virus resistance in plants related to homology-dependent gene silencing. Plant J 7: 1001-1013

    Google Scholar 

  • Palukaitis P and Zaitlin M (1997) Replicase-mediated resistance to plant virus disease. Adv Virus Res 48: 349-377

    Google Scholar 

  • Palukaitis P, Roossinck MJ, Dietzgen RG and Francki RIB (1992) Cucumber mosaic virus. Adv Virus Res 41: 281-348

    Google Scholar 

  • Price WC (1934) Isolation and study of some yellow strains of cucumber mosaic. Phytopathology 24: 743-761

    Google Scholar 

  • Rao ALN and Francki RIB (1981) Comparative studies on tomato aspermy and cucumber mosaic viruses. VI. Partial compatibility of genome segments of the two viruses. Virology 114: 573-575

    Google Scholar 

  • Rizzo TM and Palukaitis P (1990) Construction of full-length cDNA clones of cucumber mosaic virus RNAs 1, 2 and 3: Generation of infectious RNA transcripts. Mol Gen Genet 222: 249-256

    Google Scholar 

  • Roossinck MJ (1997) Mechanisms of plant virus evolution. Annu Rev of Phytopathol 35: 191-209

    Google Scholar 

  • Roossinck MJ and Palukaitis P (1990) Rapid induction and severity of symptoms in zucchini squash (Cucurbita pepo) map to RNA 1 of cucumber mosaic virus. Mol Plant-Microbe Interact 3: 188-192

    Google Scholar 

  • Roossinck MJ, Zhang L and Hellwald K-H (1999) Phylogenetic estimations of cucumber mosaic virus using the coat protein gene indicate three subgroups and radial evolution. J Virol 73: 6752-6758

    Google Scholar 

  • Sackey ST and Francki RIB (1990) Interaction of cucumoviruses in plants: persistence of mixed infections of cucumber mosaic and tomato aspermy viruses. Physiol Mol Plant Pathol 36: 409-419

    Google Scholar 

  • Scott H (1963) Purification of cucumber mosaic virus. Virology 20: 103-106

    Google Scholar 

  • Shintaku MH, Zhang L and Palukaitis P (1992) A single amino acid substitution in the coat protein of cucumber mosaic virus induced chlorosis in tobacco. Plant Cell 4: 751-757

    Google Scholar 

  • Tien P, Rao ALN and Hatta T (1982) Cucumber mosaic virus from cornflower in China. Plant Dis 66: 337-339

    Google Scholar 

  • Varveri C and Boutsika K (1999) Characterization of cucumber mosaic cucumovirus isolates in Greece. Plant Pathol 48: 95-100

    Google Scholar 

  • Zaitlin M, Anderson JM, Perry KL, Zhang L and Palukaitis P (1994) Specificity of replicase-mediated resistance to cucumber mosaic virus. Virology 201: 200-205

    Google Scholar 

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Authors and Affiliations

  1. Institute of Phytomedicine, University of Hohenheim, 70593, Stuttgart, Germany

    Karl-Heinz Hellwald

  2. Institute of Phytomedicine, University of Hohenheim, 70593, Stuttgart, Germany

    Dagmar Glenewinkel, Sonja Hauber & Sonja Wittlinger

Authors
  1. Karl-Heinz Hellwald
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  2. Dagmar Glenewinkel
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  3. Sonja Hauber
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  4. Sonja Wittlinger
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Hellwald, KH., Glenewinkel, D., Hauber, S. et al. Complementation of CMV Subgroup IA Strains in Replicase-mediated Resistant Tobacco Plants after Co-inoculation with Different Cucumoviruses. European Journal of Plant Pathology 107, 713–721 (2001). https://doi.org/10.1023/A:1011927517182

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