Characterization and wash performance analysis of an SDS-stable alkaline protease from a Bacillus sp.

  • Ruchi Oberoi
  • Qasim Khalil Beg
  • Sumant Puri
  • R.K. Saxena
  • Rani Gupta
Biography

Abstract

An alkaline, SDS-stable protease optimally active at pH 11 from a Bacillus sp. RGR-14 was produced in a complex medium containing soybean meal, starch and calcium carbonate. The protease was active over a wide temperature range of 20–80 °C with major activity between 45 and 70 °C. The protease was completely stable for 1 h in 0.1% SDS and retained 70% of its activity in the presence of 0.5% SDS after 1 h of incubation. The enzyme was active in presence of surfactants (ionic and non-ionic) with 29% enhancement in activity in Tween-85 and was also stable in various oxidizing agents with 100 and 60% activity in presence of 1% sodium perborate and 1% H2O2, respectively. The enzyme was also compatible with commercial detergents (1% w/v) such as Surf, Ariel, Wheel, Fena and Nirma, retaining more than 70% activity in all the detergents after 1 h. Wash performance analysis of grass and blood stains on cotton fabric showed an increase in reflectance (14 and 25% with grass and blood stains, respectively) after enzyme treatment. However, enzyme in conjunction with detergent proved best, with a maximum reflectance change of 46 and 34% for grass and blood stain removal, respectively, at 45 °C. Stain removal was also effective after protease treatment at 25 and 60 °C.

Alkaline protease Bacillus sp. SDS-stable wash performance 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Ruchi Oberoi
    • 1
  • Qasim Khalil Beg
    • 1
  • Sumant Puri
    • 1
  • R.K. Saxena
    • 1
  • Rani Gupta
    • 1
  1. 1.Department of MicrobiologyUniversity of DelhiNew DelhiIndia

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