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An Efficient Microtiter System to Determine Agrobacterium Biovar

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Abstract

A microtiter system for biovar characterization of Agrobacterium strains which simplifies the analysis of a large number of isolates is described. This method is based on incubation of bacterial strains in microplate wells previously amended with media specifically used by the different Agrobacterium biovars. More than 150 purified Agrobacterium strains isolated from the most common host plants were analysed by the microtiter system. It proved to be an excellent tool using less reagents, time and space for incubation in comparison with the traditional method.

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Author notes

  1. Jaime Cubero

    Present address: Citrus Research and Education Center (CREC), University of Florida, Lake Alfred, FL, 33880, USA

Authors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial 46113, Moncada, Valencia, Spain

    Jaime Cubero & María M. López

Authors
  1. Jaime Cubero
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  2. María M. López
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Correspondence to María M. López.

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Cubero, J., López, M.M. An Efficient Microtiter System to Determine Agrobacterium Biovar. European Journal of Plant Pathology 107, 757–760 (2001). https://doi.org/10.1023/A:1011906912755

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  • DOI: https://doi.org/10.1023/A:1011906912755

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