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Effect of the respiratory activity on photoinhibition of the cyanobacterium Synechocystis sp.

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Abstract

The influence of respiratory activity on photosynthesis in Synechocystis cells that had been exposed to high light intensity was studied using distinct conditions of nitrogen supply. The photoinhibitory rate of N-sufficient cells was not influenced by the presence of different nitrogen sources. In contrast, when N-starved cells were resupplied with ammonium, they were protected from photoinhibition. Although N-starved cells presented a higher rate of dark O2 uptake than N-sufficient ones, the photoinhibitory rate increased in both cases after addition of sodium azide or sodium azide plus salicylhydroxamic acid in the photoinhibitory treatment. In the absence of the D1 protein repair mechanism, photodamage to Photosystem II was faster in N-sufficient cells than in N-starved ones. Mitigation of photodamage disappeared when the respiratory activity of N-starved cells was partially suppressed by the addition of sodium azide or sodium azide and salicylhydroxamic acid. Our results suggest that electron flow through cyanobacterial terminal oxidases can assist Photosystem I in removing electrons from the reduced plastoquinone pool, thus contributing to both reopening of Photosystem II reaction centers and avoiding photogeneration of reactive oxygen species under photoinhibitory conditions.

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Authors and Affiliations

  1. Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, 21910-240, Rio de Janeiro, RJ, Brazil

    Luiz Mauro T. Silva, Cesar P. Dos Santos & Ricardo M. Chaloub

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  1. Luiz Mauro T. Silva
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  2. Cesar P. Dos Santos
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  3. Ricardo M. Chaloub
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Correspondence to Ricardo M. Chaloub.

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Silva, L.M.T., Dos Santos, C.P. & Chaloub, R.M. Effect of the respiratory activity on photoinhibition of the cyanobacterium Synechocystis sp.. Photosynthesis Research 68, 61–69 (2001). https://doi.org/10.1023/A:1011890200229

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