Skip to main content
SpringerLink
Log in

Somatic embryogenesis in Eucalyptus globulus

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Somatic embryos were obtained from 1% of cotyledon pieces and hypocotyls of mature embryos of Eucalyptus globulus Labill. cultured on media containing a high concentration of picloram or IBA. 2,4-D and other synthetic auxins did not yield somatic embryos or embryogenic callus. Somatic embryos arose indirectly via callus, being visible after four months, and directly, where little callus or adventitious root initiation occurred. Somatic embryos, formed directly from explants, were visible within five weeks. Various structural abnormalities of somatic embryos were observed, especially after induction on media containing picloram. Only two out of fifteen somatic embryos showed hypocotyl and radical elongation, but plantlets did not develop further.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Blakeway FC, Herman B & Watt MP (1993) Establishment of cell suspension cultures of Eucalyptus grandis and E. grandis × camaldulensis. Sth. Afr. J. Bot. 166: 17–26

    Google Scholar 

  • Boulay M (1987) Preliminary research on somatic embryogenesis in Eucalyptus gunnii. AFOCEL 1986 Annual Report (pp 23–27)

  • Furze MJ (1988) Somatic embryogenesis in Eucalyptus nitens. In: Forestry Research: Future Needs in Southern Africa. Absts (pp 8–9)

  • Le Roux JJ & van Staden J (1991) Micropropagation and tissue culture of Eucalyptus — a review. Tree Physiol. 9: 435–477

    Google Scholar 

  • Muralidharan EM & Mascarenhas AF (1995) Somatic embryogenesis in Eucalyptus. In: Jain SM, Gupta PK & Newton RJ (eds). Somatic Embryogenesis in Woody Plants. Vol 2 pp (23–40). Kluwer Academic Publishers. Dordrecht, Netherlands

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Ouyang Q & Peng H (1990) Eucalyptus. In: Chen Z (ed) Handbook of Plant Cell Culture, Vol 6 (pp 199–215). McGraw-Hill, New York, USA

    Google Scholar 

  • Ruaud J-N, Churchill K & Pepper S (1997) Somatic embryogenesis initiation in Eucalyptus nitens (pp 185–186). Proc. 3rd. Int'l. ISHS Symp. In vitro Culture and Horticultural Breeding, Jerusalem, Israel, 16–21 June, 1996

  • Termignoni RR, Wang P-J & Hu C-Y (1996) Somatic embryo induction in Eucalyptus dunnii. Plant Cell Tiss. Org. Cult. 45: 129–132

    Google Scholar 

  • Watt MP, Blakeway F, Cresswell CF & Herman B (1991) Somatic embryogenesis in Eucalyptus grandis. Sth. Afr. For. J. 157: 59–65

    Google Scholar 

Download references

Author information

Author notes

  1. Greg Nugent

    Present address: Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland

Authors and Affiliations

  1. Department Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, GPO Box 2476V, 3001, Melbourne, 3001, Australia

    Greg Nugent & Trevor W. Stevenson

  2. Florigene Pty. Ltd., 16 Gipps Street, Collingwood, 3066, Australia

    Stephen F. Chandler

  3. Australian Paper Plantations Pty. Ltd., P.O. Box 220, Morwell, 3840, Australia

    Phil Whiteman

Authors
  1. Greg Nugent
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Stephen F. Chandler
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Phil Whiteman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Trevor W. Stevenson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nugent, G., Chandler, S.F., Whiteman, P. et al. Somatic embryogenesis in Eucalyptus globulus. Plant Cell, Tissue and Organ Culture 67, 85–88 (2001). https://doi.org/10.1023/A:1011691110515

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1011691110515

Keywords

Search

Navigation