Abstract
HgCl2 was used at up to 10 mg l−1 as an elicitor of phytoalexins in sweet potato (Ipomoea batatas (L.) Lam. cv Centennial) cell suspension cultures. Maximum stimulation of a coumarin compound was after one day of exposure using 1 mg HgCl2 l−1. The compound was identified by HPLC and GC-MS analyses as 7-hydroxycoumarin (umbelliferone).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akazawa T,Uritani I,Akazawa Y (1962) Biosynthesis of ipomeamarone 1. The incorporation of acetate-2-14C and mevalonate-2-14C into ipomeamarone. Arch. Biochem. Biophys. 99: 52-59.
Eilert U (1987) Elicitation: methodology and aspects of application. In: Vasil IK, ed. Cell Culture and Somatic Cell Genetics of Plants. New York: Academic Press, Inc., pp. 153-196.
Gustine DL (1981) Evidence for sulfhydryl involvement in regulation of phytoalexin accumulation in Trifolium repens callus cultures. Plant Physiol. 68: 1323-1326.
Magdaleno A,Gomez CE,Velez CG,Accovinti J (1997) Preliminary toxicity tests using the green alga, Ankistrodesmus falcatus. Environ. Toxicol. Water Qual. 12: 11-14.
Modafar CE,Clerivet A,Fleuriet A,Macheix JJ (1993) Inoculation of Platanus acerifolia with Ceratocystis fimbriata F. sp. platani induces scopoletin and umbelliferone accumulation. Phytochemistry 34: 1271-1276.
Natori S,Ikekawa N,Suzuki M (1981) Abnormal secondary metabolites in plants. In: Natori S,Ikekawa N,Suzuki M, eds. Advances in Natural Products Chemistry: Extraction and Isolation of Biologically Active Compounds. New York: Halsted Press, pp. 178-194.
Oba K,Uritani I (1979) Biosynthesis of furano-terpenes by sweet potato cell culture. Plant Cell Physiol. 20: 819-826.
Oba K,Tatematsu H,Yamashita K,Uritani I (1976) Induction of furano-terpene production and formation of the enzyme system from mevalonate to isopentenyl pyrophosphate in sweet potato root tissue injured by Ceratocystis fimbriata and by toxic chemicals. Plant Physiol. 58: 51-56.
Schneider JA,Lee J,Naya Y,Nakanishi K,Oba K,Uritani I (1984) The fate of the phytoalexin ipomeamarone: furano-terpenes and butenolides from Ceratocystis fimbriata-infected sweet potatoes. Phytochemistry 23: 759-764.
Uritani I,Uritani M,Yamada H (1960) Similar metabolic alterations induced in sweet potato by poisonous chemicals and by Ceratostomella fimbriata. Phytopathology 50: 30-34.
Zhang CH,Mei XG,Liu L,Yu LJ (2000) Enhanced paclitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis. Biotechnol. Lett. 22: 1561-1564.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Smith, R.A., Drummond, S., Haines, A. et al. Induction of umbelliferone in sweet potato cell suspension culture using mercuric chloride. Biotechnology Letters 23, 1397–1400 (2001). https://doi.org/10.1023/A:1011660722402
Issue Date:
DOI: https://doi.org/10.1023/A:1011660722402