Abstract
A derivative of Klebsiella oxytoca M5A1 containing chromosomally integrated genes for ethanol production from Zymomonas mobilis (pdc, adhB) and endoglucanase genes from Erwinia chrysanthemi (celY, celZ) produced over 20 000 U endoglucanase l−1 activity during fermentation. In combination with the native ability to metabolize cellobiose and cellotriose, this strain was able to ferment amorphous cellulose to ethanol (58–76% of theoretical yield) without the addition of cellulase enzymes from other organisms.
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References
Aristidou A,Penttila M (2000) Metabolic engineering applications to renewable resource utilization. Curr. Opin. Biotechnol. 11: 187-198.
Beall, DS,Ohta K,Ingram LO (1991) Parametric studies of ethanol production from xylose and other sugars by recombinant Escherichia coli. Biotechnol. Bioeng. 38: 296-303.
Brestic-Goachet N,Gunasekaran P,Cami B,Baratti JC (1989) Transfer and expression of an Erwinia chrysanthemi cellulase gene in Zymomonas mobilis. J. Gen. Microbiol. 135: 893-902.
Chandrakant P,Bisaria VS (1998) Simultaneous bioconversion of cellulose and hemicellulose to ethanol. Crit. Rev. Biotechnol. 18: 295-331.
Cho KM,Yoo YJ (1999) Novel SSF Process for ethanol production from microcrystalline cellulose using the δ-integrated recombinant yeast, Saccharomyces cerevisiae L2612δGC. J. Microbiol. Biotechnol. 9: 340-345.
Cho KM,Yoo YJ,Kang HS (1999) δ-Integration of endo/exoglucanase and δ-glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol. Enzyme Microb. Technol. 25: 23-30.
Guiseppi A,Aymeric JL,Cami B,Barras F,Creuzet N (1991) Sequence analysis of the cellulase-encoding celY gene of Erwinia chrysanthemi: a possible case of interspecies gene transfer. Gene 106: 109-114.
He SY,Lindeberg M,Chatterjee AK,Collmer A (1991) Cloned Erwinia chrysanthemi out genes enable Escherichia coli to selectively secrete a diverse family of heterologous proteins to its milieu. Proc. Natl. Acad. Sci. USA 88: 1079-1083.
Ingram LO,Aldrich HC,Borges ACC,Causey TB,Martinez A,Morales F,Saleh A,Underwood SA,Yomano LP,York SW,Zaldivar J,Zhou S (1999) Enteric bacterial catalysts for fuel ethanol production. Biotechnol. Prog. 15: 855-866.
Lai X,Davis FC,Hespell RB,Ingram LO (1997) Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides. Appl. Environ. Microbiol. 63: 355-363.
Lynd LR,Wyman CE,Gerngross TU (1999) Biocommodity engineering. Biotechnol. Prog. 15: 777-793.
Misawa N,Okamoto T,Nakamura K (1988) Expression of a cellulase gene in Zymomonas mobilis. J. Biotechnol. 7: 167-178.
Nieves RA,Ehrman CI,Adney WS,Elander RT,Himmel ME (1998) Technical communication: survey and analysis of commercial cellulase preparations suitable for biomass conversion to ethanol. World J. Microbiol. Biotechnol. 14: 301-304.
Ogier JC,Ballerini D,Leygue JP,Rigal L,Pourquie J (1999) Ethanol production from lignocellulosic biomass. Oil Gas Technol.-Rev. Inst. Fanc. Petrol. 54: 67-94.
Pereira AN,Mobedshahi M,Ladisch MR (1988) Preparation of cellodextrins. Meth Enzymol. 160: 26-38.
Pugsley, AP,Francetic O,Possot OM,Sauvonnet N,Hardie KR (1997) Recent progress and future directions in studies of the main terminal branch of the general secretory pathway in Gramnegative bacteria. Gene 192: 13-19.
Su P,Liu CQ,Lucas RJ,Delaney SF,Dunn NW (1993) Simultaneous expression of genes encoding endoglucanase and δ-glucosidase in Zymomonas mobilis. Biotechnol. Lett. 15: 979-984.
Tomme P,Warren RAJ,Gilkes NR (1995) Cellulose hydrolysis by bacteria and fungi. Adv. Microb. Physiol. 37: 1-81.
Van Rensburg P, Van Zyl WH, Pretorius IS (1998) Engineering yeast for efficient cellulose degradation. Yeast 14: 67-76.
Van Rensburg P, Willem HVZ, Pretorius IS (1996) Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-δ-1, 4-glucanase gene in Saccharomyces cerevisiae. Curr. Genet. 30: 246-250.
Wilson DB, Spezio M, Irwin D, Karplus A, Taylor J (1997) Comparison of enzymes catalyzing the hydrolysis of in soluble polysaccharides. Amer. Chem. Soc. Symp. 618: 1-13.
Wood BE, Ingram LO (1992) Ethanol production from cellobiose, amorphous cellulose, and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum. Appl. Environ. Microbiol. 58: 2103-2110.
Wood BE, Beall, DS, Ingram LO (1997) Production of recombinant bacterial endoglucanase as aco-product with ethanol during fermentation using derivatives of Escherichia coli KO11. Biotechnol. Bioeng. 55: 547-555.
Wood TM (1988) Preparation of crystalline, amorphous, and dyed cellulase substrates. Meth Enzymol. 160: 19-25.
Zhou S, Ingram LO (1999) Engineering endoglucanase-secreting strains of ethanologenic Klebsiella oxytoca P2. J. Ind. Microbiol. Biotechnol. 22: 600-607.
Zhou S, Ingram LO (2000) Synergistic hydrolysis of carboxymethyl cellulose and acid-swollen cellulose by two endoglucanases (CelZ and CelY) from Erwinia chrysanthemi. J. Bacteriol. 182: 5676-5682.
Zhou S, Davis FC, Ingram LO (2001) Gene integration and expression, and extracellular secretion of Erwinia chrysanthemi endoglucanase CelY (celY) and CelZ (celZ) in ethanologenic Klebsiella oxytoca P2. Appl. Environ. Microbiol. 67: 6-14.
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Zhou, S., Ingram, L. Simultaneous saccharification and fermentation of amorphous cellulose to ethanol by recombinant Klebsiella oxytoca SZ21 without supplemental cellulase. Biotechnology Letters 23, 1455–1462 (2001). https://doi.org/10.1023/A:1011623509335
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DOI: https://doi.org/10.1023/A:1011623509335