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Desorption of cellulases from cotton powder

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Abstract

Cotton fabrics were treated with three different Trichoderma reesei cellulase preparations (total crude – TC, endoglucanase enriched – EG-rich, cellobiohydrolase enriched – CBH-rich) using mechanical agitation to produce cotton powder. Desorption of cellulase enzymes from the cotton powder was then performed by washing with buffer. After 3 washings most of the protein was desorbed from the cotton powder and the amount of sugars released in the latter washings was negligible. TC and CBH-rich preparations produced a finer cellulose powder than EGs. The desorption process caused a decrease in degree of polymerisation (DP) specially for the cotton treated with EGs and a marked increase in polydispersity (P d) for all preparations.

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Authors and Affiliations

  1. Department of Textile Engineering, Minho University, 4800-058, Guimarães, Portugal

    Helena Azevedo & Arthur Cavaco-Paulo

  2. Research Center in Applied Chemistry, Department of Chemistry, Federal University of Paraná, P.O. Box, 19081, Curitiba, 81531-990, Brazil

    Luiz Pereira Ramos

Authors
  1. Helena Azevedo
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  2. Luiz Pereira Ramos
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  3. Arthur Cavaco-Paulo
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Azevedo, H., Pereira Ramos, L. & Cavaco-Paulo, A. Desorption of cellulases from cotton powder. Biotechnology Letters 23, 1445–1448 (2001). https://doi.org/10.1023/A:1011619425613

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  • DOI: https://doi.org/10.1023/A:1011619425613

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