Abstract
Kluyveromyces marxianus KMDB-1, a plasmid-bearing recombinant, not carrying any particular gene of relevance, derived from auxotrophic strain KMS-2 (ura −), grew in cheese whey with a maximum specific growth rate of 0.34 h−1. This recombinant strain showed the same lactose uptake and extracellular protease production kinetics as the wild type CBS6556 with no evidence of catabolite repression. The plasmid was retained in 50% of cells after 36 h of batch culture. The presence of this vector in Kluyveromyces marxianus, which possesses no natural plasmids, together with the absence of any metabolic loading effect, creates a suitable microbial system for cheese whey processing for potential value-added product formation.
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Galvão de Souza Júnior, C., MacDonald Ledingham, W. & Antônio de Morais Júnior, M. Utilisation of cheese whey as an alternative growth medium for recombinant strains of Kluyveromyces marxianus. Biotechnology Letters 23, 1413–1416 (2001). https://doi.org/10.1023/A:1011617914709
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DOI: https://doi.org/10.1023/A:1011617914709