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The candidate gene approach in plant genetics: a review

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Abstract

The candidate gene (CG) approach has been applied in plant genetics in the past decade for the characterisation and cloning of Mendelian and quantitative trait loci (QTLs). It constitutes a complementary strategy to map-based cloning and insertional mutagenesis. The goal of this paper is to present an overview of CG analyses in plant genetics. CG analysis is based on the hypothesis that known-function genes (the candidate genes) could correspond to loci controlling traits of interest. CGs refer either to cloned genes presumed to affect a given trait (`functional CGs') or to genes suggested by their close proximity on linkage maps to loci controlling the trait (`positional CGs'). In plant genetics, the most common way to identify a CG is to look for map co-segregation between CGs and loci affecting the trait. Statistical association analyses between molecular polymorphisms of the CG and variation in the trait of interest have also been carried out in a few studies. The final validation of a CG will be provided through physiological analyses, genetic transformation and/or sexual complementation. Theoretical and practical applications of validated CGs in plant genetics and breeding are discussed.

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  1. Unité de Génétique et d'Amélioration des Fruits et Légumes, Institut National de la Recherche Agronomique (INRA), Domaine Saint-Maurice BP 94, 84143 , Montfavet cedex, France

    Stéphanie Pflieger, Véronique Lefebvre & Mathilde Causse

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  1. Stéphanie Pflieger
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Pflieger, S., Lefebvre, V. & Causse, M. The candidate gene approach in plant genetics: a review. Molecular Breeding 7, 275–291 (2001). https://doi.org/10.1023/A:1011605013259

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