Abstract
The thermal behavior associated with the melting of ice was investigated by differential scanning calorimetry (DSC) for the gel and L-subgel phases of dipalmitoylphosphatidylcholine (DPPC)-water system of varying water contents up to a full hydration. By calorimetric analysis previously developed by us, the numbers of differently bound water molecules were estimated, and used to construct water distribution diagrams (i.e., a plot of the cumulative numbers of these water molecules vs. water content) for the two phases. A comparison of the diagrams revealed the critical role of interlamellar water which changes from freezable to non-freezable one in a conversion of the gel to the L-subgel phase by the thermal annealing.
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M. J. Ruocco and G. Shipley, Biochim. Biophys. Acta, 691 (1982) 309.
M. Kodama, Thermochim. Acta, 109 (1986) 81.
M. Kodama, H. Hashigami and S. Seki, J. Colloid Interface Sci., 117 (1987) 497.
M. Kodama, H. Inoue and Y. Tsuchida, Thermochim. Acta, 266 (1995) 373.
H. Aoki and M. Kodama, J. Thermal Anal., 49 (1997) 839.
M. Kodama, H. Kato and H. Aoki, Thermochim. Acta, 352 (2000) 213.
M. Kodama, H. Aoki and T. Miyata, Biophys. Chem., 79 (1999) 205.
S. C. Chen, J. M. Sturtevent and B. J. Gaffeny, Proc. Natl. Acad. Sci. USA, 77 (1980) 5060.
H. H. Mantsch, S. C. Hsi, K. W. Butler and D. G. Cameron, Biochim. Biophys. Acta, 728 (1983) 325.
S. Mulukutla and G. G. Shipley, Biochemistry, 23 (1984) 2514.
D. A. Wilkinson and J. F. Nagle, Biochemistry, 23 (1984) 1538.
D. A. Wilkinson and T. J. McIntosh, Biochemistry, 25 (1986) 295.
R. M. Epand, B. Gabel, R. F. Epand, A. Sen, S. W. Hui, A. Muga and W. K. Surewicz, Biophys. J., 63 (1992) 327.
P. Laggner, K. Lohner, G. Degovics, K. Muller and A. Schuster, Chem. Phys. Lipids, 44 (1987) 31.
R. D. Koynova, B. G. Tenchov, P. J. Quinn and P. Laggner, Chem. Phys. Lipids, 48 (1988) 205.
M. Kodama, H. Aoki, H. Takahashi and I. Hatta, Biochim. Biophys. Acta, 1329 (1997) 61.
G. Klose, B. König, H. W. Meyer, G. Schulze and G. Degovics, Chem. Phys. Lipids, 47 (1988) 225.
J. F. Nagle, R. Zhang, T. Stephanie-Nagle, W. Sun, H. I. Petrache and R. M. Suter, Biophys. J., 70 (1996) 1419.
K. Gawrisch, W. Richter, W. A. Möps, P. Balgavy, K. Arnold and G. Klose, Studia Biophys., 108 (1985) 5.
M. Kodama, J. Nakamura, T. Miyata and H. Aoki, J. Thermal Anal., 51 (1998) 91.
H. Aoki and M. Kodama, Thermochim. Acta, 308 (1998) 77.
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Kodama, M., Kato, H. & Aoki, H. Comparison of Differently Bound Molecules in the Gel and Subgel Phases of a Phospholipid Bilayer System. Journal of Thermal Analysis and Calorimetry 64, 219–230 (2001). https://doi.org/10.1023/A:1011501516365
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DOI: https://doi.org/10.1023/A:1011501516365