Advertisement

Biomedical Microdevices

, Volume 3, Issue 3, pp 191–200 | Cite as

Development and Characterization of Microfluidic Devices and Systems for Magnetic Bead-Based Biochemical Detection

  • Jin-Woo Choi
  • Kwang W. Oh
  • Arum Han
  • C. Ajith Wijayawardhana
  • Chad Lannes
  • Shekhar Bhansali
  • Kevin T. Schlueter
  • William R. Heineman
  • H. Brian Halsall
  • Joseph H. Nevin
  • Arthur J. Helmicki
  • H. Thurman Henderson
  • Chong H. Ahn
Article

Abstract

This paper presents the development and characterization of a generic microfluidic system for magnetic bead-based biochemical detection. Microfluidic and electrochemical detection devices such as microvalves, flow sensors, biofilters, and immunosensors have been successfully developed and individually characterized in this work. Magnetically driven microvalves, pulsed-mode microflow sensors, magnetic particle separators as biofilters, and electrochemical immunosensors have been sep-arately fabricated and tested. The fabricated microfluidic components have been surface-mounted on the microfluidic motherboard for fully integrated microfluidic biochemical detection system. A magnetic bio-bead approach has been adopted for both sampling and manipulating target biological molecules. Magnetic beads were used as both substrate of antibodies and carriers of target antigens for magnetic bead-based immunoassay, which was chosen as a proof-of-concept for the generic microfluidic bio-chemical detection system. The microfluidic and electrochemical immunosensing experiment results obtained from this work have shown that the biochemical sensing capability of the complete microfluidic subsystem is suitable for portable biochemical detection of bio-molecules. The methodology and system, which has been developed in this work, can be extended to generic bio-molecule detection and analysis systems by replacing antibody/antigen with appropriate bio receptors/reagents such as DNA fragments or oligonucleotides for application towards DNA analysis and/or high throughput protein analysis.

microfluidic system magnetic bead immunoassay biochemical detection 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    C.H. Ahn, H.T. Henderson, W.R. Heineman, and H.B. Halsall, Proceedings of the Micro Total Analysis Systems '98 (μ-TAS '98) Workshop, 225-230 (1998).Google Scholar
  2. 2.
    C. Bisson, J. Campbell, R. Cheadle, M. Chomiak, J. Lee, C. Miller, C. Milley, P. Pialis, S. Shaw, W. Weiss, and C. Widrig, Technical Digest of Solid-State Sensor and Actuator Workshop 1998, pp. 1-6 (1998).Google Scholar
  3. 3.
    S. Shoji, Electronics and Communications in Japan, Part 2, 82(2), 21-29 (1999).Google Scholar
  4. 4.
    D.J. Harrison, K. Fluri, N. Chiem, T. Tang, and Z. Fan, Sensors and Actuators B 33, 105-109 (1996).Google Scholar
  5. 5.
    J. Khandurina, T.E. Mcknight, S.C. Jacobson, L.C. Waters, R.S. Foote, and J.M. Ramsey, Analytical Chemistry 72, 2995-3000 (2000).Google Scholar
  6. 6.
    J.-W. Choi, C.H. Ahn, S. Bhansali, and H.T. Henderson, Sensors and Actuators B 64, 34-39 (2000).Google Scholar
  7. 7.
    W.R. Heineman, H.B. Halsall, M. Cousino, C.A. Wijayawardhana, S. Purushothama, S. Kradtap, K. Schlueter, J.-W. Choi, C.H. Ahn, and H.T. Henderson, PITTCON '99, Orlando, FL, March 7–12, (1999).Google Scholar
  8. 8.
    D.J. Sadler, T.M. Liakopoulos, and C.H. Ahn, Proceedings of Electrochemical Society: The 5th International Symposium on Magnetic Materials, Processes, and Devices, 377-388 (1998).Google Scholar
  9. 9.
    A. Han, K.W. Oh, S. Bhansali, H.T. Henderson, and C.H. Ahn, Proceedings of IEEE MEMS '00, pp. 414-418 (2000).Google Scholar
  10. 10.
    N. Okulan, H.T. Henderson, and C.H. Ahn, Technical Digest of Solid-State Sensor and Actuator Workshop 320-323 (1998).Google Scholar
  11. 11.
    J. Pilchowski, F. Li, C.-T. Lin, T.D. Mantei, and H.T. Henderson, Proceedings of the Electrochemical Society, 193rd Meeting, San Diego, CA, May 3–8 (1998).Google Scholar
  12. 12.
    F. Bjorefors, C. Strandman, and L. Nyholm, Electroanalysis 12, 255-261 (2000).Google Scholar
  13. 13.
    O. Niwa, Y. Xu, H.B. Halsall, and W.R. Heineman, Analytical Chemistry 65, 1559-1563 (1993).Google Scholar
  14. 14.
    M. Paeschke, U. Wollenberger, T. Lisec, U. Schnakenberg, and R. Hintsche, Sensors and Actuators B 26–27, 394-397 (1995).Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Jin-Woo Choi
    • 1
  • Kwang W. Oh
    • 1
  • Arum Han
    • 1
  • C. Ajith Wijayawardhana
    • 2
  • Chad Lannes
    • 1
  • Shekhar Bhansali
    • 1
  • Kevin T. Schlueter
    • 2
  • William R. Heineman
    • 2
  • H. Brian Halsall
    • 2
  • Joseph H. Nevin
    • 1
  • Arthur J. Helmicki
    • 1
  • H. Thurman Henderson
    • 1
  • Chong H. Ahn
    • 1
  1. 1.Center for Microelectronic Sensors and MEMS, Department of Electrical and Computer Engineering and Computer ScienceUniversity of CincinnatiCincinnati
  2. 2.Department of ChemistryUniversity of CincinnatiCincinnati

Personalised recommendations