The characteristic curd of cauliflower (Brassica oleracea var. botrytis L.) consists of proliferating, arrested inflorescence and floral meristems. However, the origins and events leading to the domestication of this important crop trait remain unclear. A similar phenotype observed in the ap1-1/cal-1 mutant of Arabidopsis thaliana led to speculation that the orthologous genes from B. oleracea may be responsible for this characteristic trait. We have carried out a detailed molecular and genetic study, which allows us to present a genetic model based on segregation of recessive alleles at specific, mapped loci of the candidate genes BoCAL and BoAP1. This accounts for differences in stage of arrest between cauliflower and Calabrese broccoli (B. oleracea var. italica Plenck), and predicts the intermediate stages of arrest similar to those observed in Sicilian Purple types. Association of alleles of BoCAL-a with curding phenotypes of B. oleracea is also demonstrated through a survey of crop accessions. Strong correlations exist between specific alleles of BoCAL-a and discrete inflorescence morphologies. These complementary lines of evidence suggest that the cauliflower curd arose in southern Italy from a heading Calabrese broccoli via an intermediate Sicilian crop type. PCR-based assays for the two key loci contributing to curd development are suitable for adoption in marker-assisted selection.
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Anthony, R.G., James P.E. and Jordan B.R. 1995. The cDNA sequence of a cauliflower apetala1/squamosa homolog. Plant Physiol 108: 441–442.
Bohuon E.J.R., Keith D.J., Parkin I.A.P., Sharpe A.G. and Lydiate D.J. 1996. Alignment of the conserved C genomes of Brassica oleracea and Brassica napus. Theor. Appl. Genet. 92: 1–7.
Boukema I.W. van Hintum T.J.L. and Astley D. 1997. The creation and composition of the Brassica oleracea core collection. IPGRI Plant Genet. Res. Newsl. 111: 29–32.
Bowman J.L., Alvarez J., Weigel D., Meyerowitz E.M. and Smyth D. 1993. Control of flower development in Arabidopsis thaliana by APETALA1 and interacting genes. Development 119: 721–743.
Branca F. and Iapichino G. 1997. Some wild and cultivated Brassicaceae exploited in Sicily as vegetables. Plant Genet. Res. Newsl. 110: 22–28.
Carr S.M. and Irish V.F. 1997. Floral homeotic gene expression defines developmental arrest stages in Brassica oleracea L. vars. botrytis and italica. Planta 201: 179–188.
Clark J.M. 1988. Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucl. Acids Res. 20: 9677–9686.
Crisp P. 1982. The use of an evolutionary scheme for cauliflowers in the screening of genetic resources. Euphytica 31: 725–734.
Crisp P. and Gray A.R. 1989. Breeding old and new forms of purple heading broccoli. Cruciferae News. 9: 17–18.
Crisp P. and Tapsell C.R. 1993. Cauliflower, Brassica oleracea L. In: Kalloo G. and Bergh B.O. (eds.), Genetic Improvement of Vegetable Crops, Pergamon Press, Oxford, pp. 157–178.
De Nettancourt D. 1977. Incompatibility in Angiosperms. Springer-Verlag, Berlin.
Doyle J.J. and Doyle J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–15.
Emshwiller E. and Doyle J.J. 1998. Origins of domestication and polyploidy in oca (Oxalis tuberosa: Oxalidaceae): nrDNA ITS data. Am. J. Bot 85: 975–985.
Fujime Y. 1983. Studies on thermal conditions of curd formation and development in cauliflower and broccoli, with special reference to abnormal curd development. Mem. Fac. Agric. Kagawa Univ. 40: 117–123.
Gray A.R. 1982. Taxonomy and evolution of broccoli (Brassica oleracea var. italica. Econ. Bot. 36: 397–410.
Gray A.R. 1989. Taxonomy and evolution of broccolis and cauliflower. Baileya 23: 28–46.
Gray A.R. and Crisp P. 1979. Autumn cauliflower. Rep. Natl Veg. Res. Stn. 1978: 57–58.
Irish V.F. and Sussex I.M. 1990. Function of the apetala-1 gene during Arabidopsis floral development. Plant Cell 2: 741–753.
Kempin S.A., Savidge B. and Yanofsky M.F. 1995. Molecular basis of the cauliflower phenotype in Arabidopsis. Science 267: 522–525.
Kieffer M., Fuller M.P. and Jellings A.J. 1998. Explaining curd and spear geometry in broccoli, cauliflower and 'romanesco': quantitative variation in activity of primary meristems. Planta 206: 34–43.
Lowman A.C. and Purugganan M.D. 1999. Duplication of the Brassica oleracea APETALA1 floral homeotic gene and the evolution of domesticated cauliflower. J. Hered 90: 514–520. 613
MAFF Ministry of Agriculture, Fisheries and Food. 1998. European Community quality standards for horticultural produce: No 2 fresh vegetables, pp. 7.1-7.6.
Massie I.H. 1998. Patterns of variation in the Italian landrace cauliflower and broccoli. Ph.D. thesis, University of London.
Massie I.H., Astley D and King G.J. 1996. Patterns of genetic diversity and relationships between regional groups and populations of Italian landrace cauliflower and broccoli (Brassica oleracea L. var. botrytis L. and var. italica Plenck). Acta Hort. 407: 45–53.
Munster T., Pahnke J., DiRosa A., Kim J.T., Martin W., Saedler H. and Theissen G. 1997. Floral homeotic genes were recruited from homologous MADS-box genes pre-existing in the common ancestor of ferns and seed plants. Proc. Natl. Acad. Sci. USA 94: 2415–2420.
Olsen K.M. and Schaal B.A. 1999. Evidence on the origin of cassava: Phylogeography of Manihot esculenta. Proc. Natl. Acad. Sci. USA 96: 5586–5591.
Pease M.S. 1996. Genetic studies in Brassica oleracea. J. Genet. 16: 363–385.
Purugganan M.D., Lowman A.C. and Suddith J. 2000. Variation and selection at the CAULIFLOWER floral homeotic gene accompanying the evolution of domesticated Brassica oleracea. Genetics 155: 855–862.
Rong-Lin W., Stec A., Hey J., Lukens L. and Doebley J. 1999. The limits of selection during maize domestication. Nature 398: 236–239.
Sadik S. 1962. Morphology of the curd of cauliflower. Am. J. Bot 49: 290–297.
Sebastian R.L., Howell E.C., King G.J., Marshall D.F. and Kearsey M.J. 1999. An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled haploid mapping populations. Theor. Appl. Genet 100: 75–81.
Shannon S. and Meeks-Wagner D.R. 1993. Genetic interactions that regulate inflorescence development in Arabidopsis. Plant Cell 5: 639–655.
Slocum M.K., Figdore S.S., Kennard W.C., Suzuki J.Y. and Osborn J.C. 1990. Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor. Appl. Genet 80: 57–64.
Stam P. and van Ooijen J.W. 1995. JoinMapTM version 2.0; Software for the calculation of genetic linkage maps. CPRODLO, Wageningen.
Thompson K.F. 1976. Cabbages, kales etc. Brassica oleracea (Cruciferae). In: Simmonds N.W. (ed.), Evolution of Crop Plants, Longman, London, pp. 49–52.
Watts L.E. 1965. Investigations on the inheritance and responses to selection of riceyness in early summer cauliflower. Euphytica 15: 111–115.
Weigel D., Alvarez J., Smyth D.R., Yanofsky, M.F. and Meyerowitz E.M. 1992. LEAFY controls floral meristem identity in Arabidopsis; Cell 69: 843–859.
Yeager A.E. 1943. The characteristics of crosses between botanical varieties of cabbage, Brassica oleracea. Proc. Am. Soc. Hort. Sci. 43: 199–200.
Zeven A.C. 1996. Sixteenth to eighteenth century depictions of cole crops (Brassica oleracea L.), turnips (B. rapa L. cultivar group vegetable turnip) and radish (Raphanus sativus L.) from Flanders and the present day Netherlands. Acta Hort. 407: 29–33.
Zhou X., Jellen E.N. and Murphy J.P. 1999. Progenitor germplasm of domesticated hexaploid oat. Crop. Sci. 39: 1208–1214
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Smith, L.B., King, G.J. The distribution of BoCAL-a alleles in Brassica oleracea is consistent with a genetic model for curd development and domestication of the cauliflower. Molecular Breeding 6, 603–613 (2000). https://doi.org/10.1023/A:1011370525688
- MADS box genes
- marker-assisted selection