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Synthesis, Immunogenicity, and Antigenicity of the Glycoprotein E Fragments of the Tick-Borne Encephalitis Virus

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Abstract

Six peptide fragments of the envelope protein E of the tick-borne encephalitis virus involving the predicted T-helper epitopes were synthesized. Their ability to induce antibodies without conjugation with any high-molecular-mass carrier was studied in mice of three lines. Five of six synthesized peptides exhibited immunogenic properties, which differed in dependence on the haplotype of immunized mice. The peptide binding to the antiviral antibodies was studied, and two peptides were revealed that demonstrated a high ability to recognize the viral antibodies in the horse and human sera. These peptides are promising for the development of diagnostic agents for the tick-borne encephalitis virus.

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Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997, Russia

    T. D. Volkova, O. M. Vol'pina, M. N. Zhmak & V. T. Ivanov

  2. Chumakov Institute of Poliomyelitis and Viral Encephalitis, Russian Academy of Sciences, Moscow, 142782, Russia

    M. F. Vorovich & A. V. Timofeev

Authors
  1. T. D. Volkova
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  2. O. M. Vol'pina
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  3. M. N. Zhmak
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  4. V. T. Ivanov
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  5. M. F. Vorovich
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  6. A. V. Timofeev
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Volkova, T.D., Vol'pina, O.M., Zhmak, M.N. et al. Synthesis, Immunogenicity, and Antigenicity of the Glycoprotein E Fragments of the Tick-Borne Encephalitis Virus. Russian Journal of Bioorganic Chemistry 27, 151–155 (2001). https://doi.org/10.1023/A:1011321018814

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  • DOI: https://doi.org/10.1023/A:1011321018814

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