Skip to main content
SpringerLink
Log in

Biological properties of copolymer of 2-Hydroxyethyl methacrylate with sulfopropyl methacrylate

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Interaction of organism with non-toxic implanted polymers depends on the physicochemical properties of the implant surface, which influence the adsorption of bioactive proteins and subsequently adhesion and growth of cells. The synthetic hydrogels are known as poorly adhesive surfaces. In this study we demonstrated the adsorption of albumin, fibrinogen, fibronectin, basic fibroblast growth factor, heparin-binding epidermal growth factor-like growth factor and epidermal growth factor to poly(2-hydroxyethyl methacrylate) (pHEMA) and copolymer of 2-hydroxyethyl methacrylate (HEMA) and potassium salt of 3-sulfopropyl methacrylate (SPMAK). The adhesion and growth of 3T3 cells and human keratinocytes on surface of these polymers was tested without and with pretreatment of polymers with heparin-binding epidermal growth factor-like growth factor. The adhesion of mixture of human granulocytes and monocytes to these surfaces was also tested. The strips of both polymers were subcutaneously and intracerebrally implanted into the rat and the extent of foreign body reaction and brain biocompatibility was evaluated. The results showed the extensive adsorption of basic fibroblast growth factor and heparin-binding epidermal growth factor-like growth factor to copolymer containing SPMAK. However the adhesion (and growth) of cells to this type of copolymers was very low. Preadsorption of human plasma to pHEMA clearly stimulated the leukocyte adhesion in contrary to copolymer containing SPMAK. The extent of foreign-body reaction was significantly higher against the pHEMA compared to tested copolymer p(HEMA-co-SPMAK). In conclusion, the tested copolymer was a poorly adhesive substrate that is only poorly recognized by the non-specific immunity, although the adsorption of basic growth factors to this substrate is highly significant. Both polymers were well tolerated by the brain tissue. The phenotype of surrounding neurons was more close to the control neurons in the brain tissue surrounding the p(HEMA-co-SPMAK) implants. © 2001 Kluwer Academic Publishers

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. K. Smetana Jr., Biomaterials 14 (1993) 1046.

    Google Scholar 

  2. J. E. Babensee, J. M. Anderson, L. V. Mcintire and A. G. Mikos, Adv. Drug Delivery Rev. 33 (1998) 111-139.

    Google Scholar 

  3. L. Tang and J. W. Eaton, J. Exp. Med. 178 (1993) 2147.

    Google Scholar 

  4. L. Tang and J. W. Eaton, Cells Mater. 4 (1994) 429.

    Google Scholar 

  5. L. Tang and J. W. Eaton, Am. J. Clin. Pathol. 103 (1995) 466.

    Google Scholar 

  6. A. K. Mcnally and J. M. Anderson, Proc. Natl. Acad. Sci. USA 91 (1994) 10119.

    Google Scholar 

  7. J. M. Anderson, N. P. Ziats, A. Azeez, M. R. Brunstedt, S. Stack and T. L. Bonfield, J. Biomater. Sci. Polymer Edn. 7 (1995) 159.

    Google Scholar 

  8. K. Smetana Jr., M. JelÍnkovÁ, J. VacÍk and M. NovÁk, J. Mater. Sci. Mater. Med. 6 (1995) 32.

    Google Scholar 

  9. F. Grinnell and M. K. Feld, J. Biol. Chem. 257 (1982) 4888.

    Google Scholar 

  10. K. Smetana, Jr., J. VacÍk, D. SouČkovÁ, Z. KrČovÁ and J. Šulc, J. Biomed. Mater. Res. 24 (1990) 463.

    Google Scholar 

  11. M. JirouŠkovÁ, J. BartÚŇkovÁ, K. Smetana Jr., J. LukÁŠ, J. VacÍl and J. E. Dyr, J. Mater. Sci. Mater. Med. 8 (1997) 19.

    Google Scholar 

  12. K. Smetana Jr., J. LukÁŠ, V. PaleČkovÁ, J. BartÚŇkovÁ, E.-T. Liu, J. VacÍk and H.-J. Gabius, Biomaterials 18 (1997) 1009.

    Google Scholar 

  13. K. Smetana, Jr., J. VacÍk, M. Houska, D. SouČkovÁ, J. LukÁŠ Macrophage recognition of polymers: effect of carboxylate groups. J. Mater. Sci. Mater. Med. 4 (1993) 526-529.

    Google Scholar 

  14. K. Smetana Jr., J. VacÍk, D. SouČkovÁ and Š. PitrovÁ, Clin. Mater. 13 (1993) 47.

    Google Scholar 

  15. S. ŠevČÍk, J. VacÍk, D. ChmelÍkovÁ and K. Smetana Jr., J. Mater. Sci. Mater. Med. 6 (1995) 505.

    Google Scholar 

  16. H. Kresse, in “Glycosciences. Status and Perspectives”, edited by H.-J. Gabius and S. Gabius. (Chapman & Hall, London) pp. 201-222.

  17. K. Smetana Jr., Exp. Molec. Pathol. 46 (1987) 258.

    Google Scholar 

  18. E. FifkovÁ and J. MarŠala, in “Electrophysiological Methods in Biological Research”, edited by J. Bureš M. Petráň and J. Zachar (Prague, Pub. House of Czechoslovak. Acad. Sci., 1967) pp. 653-731.

    Google Scholar 

  19. L. W. Swanson, “Brain Maps: Structure of the Rat Brain” (Elsevier, Amsterdam, London, New York, Tokyo, 1992).

    Google Scholar 

  20. U. Scherer-Singler, S. R. Vincent, H. Kimura and E. G. Mcgeer, J. Neurosci. Meth. 9 (1983) 229.

    Google Scholar 

  21. P. PetrovickÝ and V. NĚmcovÁ, J. Brain Res. 36 (1995) 539.

    Google Scholar 

  22. P. B. Gordon, H. U. Choi, G. Conn, A. Ahmed, B. Ehrmann, L. Rosenberg and V. Hatcher, J. Cell. Physiol. 140 (1989) 584.

    Google Scholar 

  23. E. Ruoslahti, Y. Yamaguchi, A. Hildebrand and W. A. Border, Cold Spring Harbor Symp. Quant. Biol. 57 (1992) 309.

    Google Scholar 

  24. C. J. Dowd, C. L. Cooney and M. A. Nugent, J. Biol. Chem. 274 (1999) 5236.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Jaromir Lukáš, Karel Smetana, Věra Palečková & Jiří Vacik

  2. 1st Faculty of Medicine, Institute of Anatomy, Charles University, U nemocnice 3, 128 00, Prague 2, Czech Republic

    Karel Smetana, Pavel Petrovický & Zuzana Holíková

  3. 3rd Faculty of Medicine, Prague Burns Center, Charles University, Prague, Czech Republic

    Barbora Dvořánková & Ludomír Brož

  4. 2nd Faculty of Medicine, Institute of Immunology, Charles University, Prague, Czech Republic

    Dagmar Pospíšilová & Jiřina Bartůňková

Authors
  1. Jaromir Lukáš
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karel Smetana
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pavel Petrovický
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Věra Palečková
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jiří Vacik
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Barbora Dvořánková
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ludomír Brož
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Dagmar Pospíšilová
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Zuzana Holíková
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Jiřina Bartůňková
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Karel Smetana.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lukáš, J., Smetana, K., Petrovický, P. et al. Biological properties of copolymer of 2-Hydroxyethyl methacrylate with sulfopropyl methacrylate. Journal of Materials Science: Materials in Medicine 12, 639–646 (2001). https://doi.org/10.1023/A:1011297828955

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1011297828955

Keywords

Search

Navigation