Abstract
Root rot of cocoyam (Xanthosoma sagittifolium) caused by Pythium myriotylum is the most devastating disease of this important tropical tuber crop with yield reductions of up to 90%. Bioassays were conducted in vitro and in sterile volcanic soil artificially infested with Pythium myriotylum, isolate CRPm, to test whether Pseudomonas aeruginosa PNA1 can control the cocoyam root rot disease. P. aeruginosa PNA1 (wild type) produces phenazine-1-carboxylic acid and phenazine-1-carboxamide (oxychlororaphin), while its tryptophan auxotrophic mutant FM13 is phenazine negative and secretes anthranilate in vitro. PNA1 and FM13 have previously been shown to control Pythium debaryanum and Pythium splendens on lettuce and bean. PNA1 and FM13 significantly inhibited growth of P. myriotylum in dual cultures, while their supernatants highly reduced mycelial dry weight in potato dextrose broth. However, in the presence of tissue culture derived cocoyam plantlets, only strain PNA1 strongly reduced root rot disease severity. Soil experiments involving strain PNA1 in comparison to phenazine-deficient mutants suggested that the biocontrol activity of PNA1 against P. myriotylum may involve phenazines. Phenazine involvement was further strengthened by the fact that FM13 fed with exogenous tryptophan (so that phenazine production is restored) significantly reduced disease severity on cocoyam. The efficiency of PNA1 to control P. myriotylum on cocoyam was significantly improved when the strain and the pathogen were allowed to interact for 24 h prior to transplanting cocoyam plantlets, while doubling the inoculum density of the pathogen negatively affected its efficiency.
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References
Anjaiah V (1998) Molecular analysis of biological control mechanisms of a fluorescent Pseudomonas aeruginosa strain PNA1, involved in the control of plant root diseases. Ph.D. thesis, Vrije Universiteit Brussel, Belgium
Anjaiah V, Koedam N, Nowak-Thompson B, Loper JE, Höfte M, Tambong JT and Cornelis P (1998) Involvement of phenazines and anthranilate in the antagonism of Pseudomonas aeruginosa PNA1 and Tn5 derivatives toward Fusarium spp. and Pythium spp. Molecular Plant-Microbe Interactions 11: 847-854
Buysens S, Heungens K, Poppe J and Höfte M (1996) Involvement of pyochelin and pyoverdin in suppression of Pythium-induced damping-off of tomato by Pseudomonas aeruginosa 7NSK2. Applied and Environmental Microbiology 62: 865-871
Chin-A-Woeng TFC, Bloemberg GV, van der Drift KMGF, Schripsema J, Kroon B, Scheffer RJ, Keel C, Bakker PAHM, Tichy HV, de Bruijn FJ, Thomas-Oates JE and Lugtenberg BJJ (1998) Biocontrol by phenazine-1-carboxamide-producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f. sp. radicis-lycopersici. Molecular Plant-Microbe Interactions 11: 1069-1077
Dowling DN and O'Gara F (1994) Metabolites of Pseudomonas involved in the biocontrol of plant disease. Trends in Biotechnology 12: 133-141
Duffy BK and Defago G (1999) Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains. Applied and Environmental Microbiology 65: 2429-2438
Essar DW, Eberly L, Chun-ya-han and Crawford IP (1990a) DNA sequences and characterisation of four early genes of the tryptophan pathway in Pseudomonas aeruginosa. Journal of Bacteriology 172: 853-866
Essar DW, Eberly L, Hadero A and Crawford IP (1990b) Identi-fication and characterisation of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications. Journal of Bacteriology 172: 884-900
Gamborg OL, Miller RA and Ojima K (1968) Nutrient requirements for suspension cultures of soybean root cells. Experimental Cell Research 50: 151-158
Giacometti DC and León J (1994) Tannia, Yautia (Xanthosoma sagittifolium). In: Hernándo JE, Bermejo JE and León J (eds) Neglected crops: 1492 from a different perspective. Plant Production and Protection Series 26: 253-258 FAO, Rome, Italy
Hamdan H, Weller DM and Thomashow LS (1991) Relative importance of fluorescent siderophores and other factors in biological control of Gaeumannocyces graminis var. tritici by Pseudomonas fluorescens 2-79 and M4-80R. Applied and Environmental Microbiology 57: 3270-3277
Haynes WC, Stodola FH, Locke JM, Pridham TG, Conway HF, Sohns VE and Jackson WR (1956) Pseudomonas aureofaciens kluyver and phenazine α-carboxylic acid, its characteristic pigment. Journal of Bacteriology 72: 412-417
Latifi A, Foglino M, Tanaka K, Williams P and Lazdunski A (1996) A hierarchical quorum-sensing cascade in Pseudomonas aeruginosa links the transcriptional activators LasR and RhlR (VsmR) to expression of the stationaryphase sigma factor RpoS. Molecular Microbiology 21: 1137-1146
Liu L, Kloepper JW and Tuzun S (1996) Induction of systemic resistance in cucumber against Fusarium wilt by plant growthpromoting rhizobacteria. Phytopathology 85: 695-698
Manch JN and Crawford IP (1982) Genetic evidence for a positive-acting regulatory factor mediating induction in the tryptophan pathway of Pseudomonas aeruginosa. Journal of Molecular Biology 156: 67-77
Martin FN and Loper JE (1999) Soil-borne plant diseases caused by Pythium spp.: Ecology, Epidemiology, and prospects for biological control. Critical Reviews in Plant Science 18: 111-181
Mavrodi DV, Ksenzenko VN, Bonsall RF, Cook RJ, Boronin AM and Thomashow LS (1998) A seven-gene locus for synthesis of phenazine-1-carboxylic acid by Pseudomonas fluorescens 2-79. Journal of Bacteriology 180: 2541-2548
Montesinos E and Bonaterra A (1996) Dose-response models in biological control of plant pathogens: an emperical verification. Phytopathology 86: 464-472
Nzietchueng S (1983) La pourriture racinaire du macabo (Xanthosoma sagittifolium) au Cameroun: I. Symptomatologie de la maladie. Agronomie Tropicale 38: 321-325
O'sullivan DJ and O'Gara FO (1992) Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens. Microbiological Reviews 56: 662-676
Ownley BH, Weller DM and Thomashow LS (1991) Influence of in situ and in vitro pH on suppression of Gaeumannocyces graminis var. tritici by Pseudomonas fluorescens 2-79. Phytopathology 82: 178-184
Pacumbaba RP, Wutoh JG, Sama AE, Tambong JT and Nyochembeng LM (1992) Isolation and pathogenecity of rhizosphere fungi of cocoyam in relation to cocoyam root rot disease. Journal of Phytopathology 135: 265-273
Pierson LS III, Gaffney T, Lam S and Gong F (1995) Molecular analysis of genes encoding phenazine biosynthesis in the biological control bacterium Pseudomonas aureofaciens 30-84. FEMS Microbiology Letters 134: 299-307
SPSS (1996) SPSS for Windows Release 7.5. Chicago: SPSS Inc
Stead P, Rudd BAM, Bradshaw H, Noble D and Dawson MJ (1996) Induction of phenazine biosynthesis in cultures of Pseudomonas aeruginosa by L-N-(3-oxohexanoyl) homoserine lactone. FEMS Microbiology Letters 140: 15-22
Tambong JT, Poppe J and Höfte M (1999) Pathogenicity, electrophoretic characterisation and in planta detection of the cocoyam root rot disease pathogen, Pythium myriotylum. European Journal of Plant Pathology 105: 597-607
Tambong JT, Sapra VT and Garton S (1998) In vitro induction of tetraploid in colchicine-treated cocoyam plantlets. Euphytica 104: 191-197
Thomashow LS and Weller DM (1996) Current concepts in the use of introduced bacteria for biological disease control: Mechanisms and antifungal metabolites. In: Stacey G and Keen NT (eds) Plant-Microbe Interactions Vol I (pp 187-235) Chapman and Hall, New York
Thomashow LS, Weller DM, Bonsall RF and Pierson LS III (1990) Production of the antibiotic phenazine-1-carboxylic acid by fluorescent Pseudomonas species in the rhizosphere of wheat. Applied and Environmental Microbiology 56: 908-912
Timms-Wilson TM, Ellis RJ, Renwick A, Rhodes DJ, Mavrodi DV, Weller DM, Thomashow LS and Bailey MJ (2000) Chromosomal insertion of phenazine-1-carboxylic acid biosynthetic pathway enhances efficacy of damping-off disease control by Pseudomonas fluorescens. Molecular Plant- Microbe Interactions 13: 1293-1300
Turner JM and Messenger AJ (1986) Occurrence, biochemistry and physiology of phenazine pigment production. Advances in Microbial Physiology 27: 211-275
Weller DM (1988) Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annual Review of Phytopathology 73: 379-407
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Tambong, J.T., Höfte, M. Phenazines are Involved in Biocontrol of Pythium myriotylum on Cocoyam by Pseudomonas aeruginosa PNA1. European Journal of Plant Pathology 107, 511–521 (2001). https://doi.org/10.1023/A:1011274321759
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DOI: https://doi.org/10.1023/A:1011274321759