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Side chain mobility as monitored by CH-CH cross correlation: The example of cytochrome b5

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Abstract

The mobility of βCH2 moieties in oxidized and reduced cytochrome b 5 was studied by analyzing the 13C relaxation of the J-split components, in terms of C-H dipole–C-H dipole cross correlation rates. A 2D 13C-1H experiment is proposed to measure these rates that provide the internal effective reorientation correlation time for each CH2 moiety. It is found that higher mobility is present in the α helices forming the heme pocket. On the contrary, the β strands, which form the hydrophobic core of the molecule, have the lowest mobility. The general pattern is the same for the oxidized and reduced species, indicating that any oxidation-dependent property detected for backbone NH moieties does not affect the CH2 mobility.

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Authors
  1. Lucia Banci
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  2. Ivano Bertini
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  3. Isabella C. Felli
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  4. Parvana Hajieva
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  5. Maria Silvia Viezzoli
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Banci, L., Bertini, I., Felli, I.C. et al. Side chain mobility as monitored by CH-CH cross correlation: The example of cytochrome b5. J Biomol NMR 20, 1–10 (2001). https://doi.org/10.1023/A:1011245101351

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