Abstract
We assessed the genetic differentiation of the Mediterranean olive from its wild relatives found in different geographic areas (Mediterranean, Asia, Africa) using eighty RAPDs revealed with eight primers. Variance analysis (AMOVA) enabled us to estimate the overall genetic differentiation parameters between wild populations. Oleasters from the Near East and Turkey were discriminated from the other Mediterranean populations. Olea laperrinei, O. maroccana and O. cerasiformis were the taxa the most related to the Mediterranean olive. In contrast, O. africana was shown to be the most genetically distant taxa from the Mediterranean olive. However, we characterised hybrid trees between these two taxa. Significant trends between genetic and geographic distances were met within the subspecies cuspidata and within the Mediterranean olive. A genetic diversity gradient was observed in both subspecies europaea and cuspidata. These results are in agreement with a mechanism of differentiation by distance in the O. europaea complex, but another non-exclusive mechanism could also be gene flow between differentiated taxa. Furthermore, we characterised the discriminating power of each RAPD to recognise the different taxa using intraclass correlation coefficients. Lastly, IGS-RFLPs enabled us to assess rDNA polymorphisms on a sub-sample of individuals. On the basis of these data, a low interspecifc differentiation was found. This suggests a recent genetic divergence between the different taxa of the O. europaea complex or the occurrence of gene flow during favourable periods or because human displacements. All the olive cultivars were genetically related to the oleaster populations supporting that Mediterranean is the olive domestication area.
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References
Angiolillo A., Mencuccini M. and Baldoni L. 1999. Olive genetic diversity assessed using amplified fragment length polymorphisms Theor. Appl. Genet. 98: 411-421.
Baradat P. and Labbé T. 1995. OPEP: Un logiciel intégré pour l'amélioration des plantes pérennes. In Traitements statistiques des essais de sélection. Stratégies de sélection des plantes pérennes, CIRAD-CP (Ed.), pp. 303-330. Montpellier.
Benzécri J.P. 1973. L'analyse des données. Tome I. La Taxonomie. Eds. Dunod, Paris.
Besnard G. 1999. Étude de la diversité génétique de l'olivier cultivé et de ses formes sauvages apparentées à l'aide de marqueurs moléculaires: applications pour l'identification variétale et pour la gestion des ressources génétiques. These Université Montpellier II, 174 pages.
Besnard G. and Bervillé A. 2000. Multiple origins for Mediterranean olive (Olea europaea L. subsp. europaea) based upon mitochondrial DNA polymorphisms. C.R. Acad. Sci., Paris, Sér III, 323: 178-181.
Besnard G., Green P.S. and Bervillé A. Taxonomic revision of the genus Olea using molecular approaches. (Submitted).
Besnard G., Khadari B., Villemur P. and Bervillé A. 2000. Cytoplasmic male sterility in the olive (Olea europaea L.). Theor. Appl. Genet. 100: 1018-1024.
Bitonti M.B., Cozza R., Chiappetta A., Contento A., Minelli S., Ceccarelli M., Gelati M.T., Maggini F., Baldoni L. and Cionini P.G. 1999. Amount and organization of the heterochromatin in Olea europaea and related species. Heredity 83: 188-195.
Browicz K. and Zielinski J. 1990. Chorology of trees and shrubs in south-west Asia and adjacent regions. Polish Scientific Publishers 7: 13-15.
Chevalier A. 1948. L'origine de l'olivier cultivé et ses variations. Rev. Int. Bot. App. Agric. Trop. 28:1-25.
de la Cruz M., Whitkus R., Gomez-Pompa A. and Mota-Bravo L. 1995. Origins of cacao cultivation. Nature 375: 542-543.
Durham R.E. and Korban S.S. 1994. Evidence of gene introgression in apple using RAPD markers. Euphytica 79: 109-114.
El Mousadik A. and Petit R.J. 1996. Chloroplast DNA phylogeography of the argan tree of Morocco. Mol. Ecol. 5: 547-555.
Epperson B.K. 1990. Spatial patterns in genetic variation within plant populations. In: Brown, Clegg, Kahler & Weir (Eds.), Plant Population, Genetics, Breeding and Genetic Resources, pp. 229-277. Sinauer, Sunderland (Mass.).
Excoffier L., Smouse P.E. and Quattro J.M. 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479-491.
Goldsbrough P.B. and Cullis C.A. 1981. Characterization of the genes for ribosomal RNA in flax. Nucleic Acids Res. 8: 4851-4855.
Green P.S. and Wickens G.E. 1989. The Olea europaea complex. The Davis & Hedge Festschrift, ed. Kit Tan, pp.287-299. Edinburgh University Press.
Hewitt G.M. 1996. Some genetic consequences of ice ages, and their role in divergence and speciation. Biol. J. Linn. Soc. 58: 247-276.
Jeandroz S., Pugin A. and Bervillé A. 1996. Cloning and analysis of a 6.8-kb rDNA intergenic spacer region of the European ash (Fraxinus excelsior L.). Theor. Appl. Genet. 92: 1003-1008.
Kabbaj A., Zeboudj F., Peltier D., Tagmount A., Tersac M., Dulieu H. and Bervillé A. 1995. Variation and phylogeny of the ribosomal DNA unit types and 5 S DNA in Petunia. Genet. Res. Crop Evol. 42: 311-325.
Lashermes P., Cros J., Marmey P. and Charrier A. 1993. Use of random amplified DNA markers to analyse genetic variability and relationships of Coffea species. Genet. Res. Crop Evol. 40: 91-99.
Lashermes P., Andrzejewski S., Bertrand B., Combes M.C., Dussert S., Graziosi G., Trouslot P. and Anthony F. 2000. Molecular analysis of introgressive breeding in coffee (Coffea arabica L.). Theor. Appl. Genet. 100:139-146.
Lebart L., Morineau A. and Piron M. 1997. Statistique exploratoire multidimensionnelle. 2nd edition. Ed. Dunod, Paris, 439 pp.
Luo H., Van Coppenolle B., Seguin M. and Boutry M. 1995. Mitochondrial DNA polymorphism and phylogenetic relationships in Hevea brasiliensis. Mol. Breed. 1: 51-63.
Maley J. 1980. Les changements climatiques de la fin du Tertiaire en Afriqu: Leur conséquence sur l'apparition du Sahara et de sa végétation. In: The Sahara and the Nile, M.A.J. William & H. Faure (Eds.), pp. 63-86. AA Balkema, Rotterdam.
Nei M. and Li W.H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA. 92:6720-6722.
Quézel P. 1978. Analysis of the flora of Mediterranean and Sahara Africa. Ann. Mo. Bot. Gard. 65: 479-534.
Quillet M.C., Madjidian N., Griveau Y., Serieys H., Tersac M., Lorieux M. and Bervillé A. 1995. Mapping genetic factors controlling pollen viability in an interspecific cross in Helianthus sect. Helianthus. Theor. Appl. Genet. 91: 1195-1202.
Rogers S.O. and Bendich A.J. 1987. Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. Plant Mol. Biol. 9:509-520.
Saitou N. and Nei M. 1987. The Neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
Sambrook J., Fritsch E.F. and Maniatis T. 1989. Molecular cloning. Second edition. Cold Spring Harbor Laboratory Press.
Santoni S. and Bervillé A. 1992. Characterization of the nuclear ribosomal DNA units and phylogeny of Beta L. wild forms and cultivated beets. Theor. Appl. Genet. 83: 533-452.
Saporta G. 1990. Probabilité, analyse des données et statistique. TECHNIP Ed., Paris, 493 pp.
Shao J. and Tu D. 1995. The Jackknife and the Bootstrap. Springer Ed., New York, 516 pp.
Terral J.F. and Arnold-Simard G. 1996. Beginnings of olive cultivation in Eastern Spain in relation to Holocene bioclimatic changes. Quaternary Res. 46: 176-185.
Tremousaygue D., Grellet F., Delseny M., Delourme R. and Renard M. 1988. The large spacer of a nuclear ribosomal RNA gene from radish: organization and use as a probe in rapeseed breeding. Theor. Appl. Genet. 75: 298-3O4.
Turrill W.B. 1951. Wild and cultivated olives.Kew Bull. 3: 437-442.
Zohary D. 1994. The wild genetic resources of the cultivated olive. Acta Hort. 356: 62-65.
Zohary D. and Hopf M. 1994. Domestication of plants in the Old World. Second edition. Oxford Clarendon Press, pp. 137-442.
Zohary D. and Spiegel-Roy P. 1975. Beginnings of fruit growing in the Old World. Science 187: 319-327.
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Besnard, G., Baradat, P., Chevalier, D. et al. Genetic differentiation in the olive complex (Olea europaea) revealed by RAPDs and RFLPs in the rRNA genes. Genetic Resources and Crop Evolution 48, 165–182 (2001). https://doi.org/10.1023/A:1011239308132
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DOI: https://doi.org/10.1023/A:1011239308132