Journal of Biomolecular NMR

, Volume 20, Issue 2, pp 135–147 | Cite as

Improved 3D triple resonance experiments, HNN and HN(C)N, for HN and 15N sequential correlations in (13C, 15N) labeled proteins: Application to unfolded proteins

  • Sanjay C. Panchal
  • Neel S. Bhavesh
  • Ramakrishna V. Hosur


Two triple resonance experiments, HNN and HN(C)N, are presented which correlate HN and 15N resonances sequentially along the polypeptide chain of a doubly (13C, 15N) labeled protein. These incorporate several improvements over the previously published sequences for a similar purpose and have several novel features. The spectral characteristics enable direct identification of certain triplets of residues, which provide many starting points for the sequential assignment procedure. The experiments are sensitive and their utility has been demonstrated with a 22 kDa protein under unfolding conditions where most of the standard triple resonance experiments such as HNCA, CBCANH etc. have limited success because of poor amide, Cα and Cβ chemical shift dispersions.

HN(C)N HNN sequential 15N correlations triple resonance experiments triplets unfolded proteins 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Sanjay C. Panchal
    • 1
  • Neel S. Bhavesh
    • 1
  • Ramakrishna V. Hosur
    • 1
  1. 1.Department of Chemical SciencesTata Institute of Fundamental ResearchMumbaiIndia

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