Abstract
A new TROSY relayed HCCH-COSY pulse sequence is introduced for correlating adenine H2 and H8 resonances in 13C-labeled RNA molecules. The pulse scheme provides substantial improvements in signal-to-noise compared to previously suggested experiments, and therefore will be suitable for NMR studies of larger RNA molecules. The experiment provides 13C chemical shifts for all carbon nuclei in the adenine base. This is advantageous for resolving spectral overlap in larger RNA molecules and provides a starting point for measuring additional parameters for these carbons in the adenine spin system.
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Batey, R.T., Inada, M., Kujawinski, E., Puglisi, J.D. and Williamson, J.R. (1992) Nucleic Acids Res., 20, 4515-4523.
Brutscher, B., Boisbouvier, J., Pardi, A., Marion, D. and Simorre, J.-P. (1998) J. Am. Chem. Soc., 120, 11845-11851.
Farmer II, B.T., Müller, L., Nikonowicz, E.P. and Pardi, A. (1993) J. Am. Chem. Soc., 115, 11040-11041.
Fiala, R., Czernek, J. and Sklenar, V. (2000) J. Biomol. NMR, 16, 291-302.
Fiala, R., Jiang, F. and Patel, D.J. (1996) J. Am. Chem. Soc., 118, 689-690.
Geen, H. and Freeman, R. (1991) J. Magn. Reson., 93, 93-141.
Heus, H.A., Wijmenga, S.S., van de Ven, F.J.M. and Hilbers, C.W. (1994) J. Am. Chem. Soc., 116, 4983-4984.
Ippel, J.H., Wijmenga, S.S., de Jong, R., Heus, H.A., Hilbers, C.W., de Vroom, E., van der Marel, G.A. and van Boom, J.H. (1996) Magn. Reson. Chem., 34, S156-S176.
Legault, P., Farmer II, B.T., Müller, L. and Pardi, A. (1994) J. Am. Chem. Soc., 116, 2203-2204.
Marino, J.P., Prestegard, J.H. and Crothers, D.M. (1994a) J. Am. Chem. Soc., 116, 2205-2206.
Marino, J.P., Schwalbe, H., Anklin, C., Bermel, W., Crothers, D.M. and Griesinger, C. (1994b) J. Am. Chem. Soc., 116, 6472-6473.
Meissner, A. and Sø rensen, O.W. (1999a) J. Magn. Reson., 139, 447-450.
Meissner, A. and Sø rensen, O.W. (1999b) J. Magn. Reson., 139, 439-442.
Nikonowicz, E.P., Sirr, A., Legault, P., Jucker, F.M., Baer, L.M. and Pardi, A. (1992) Nucleic Acids Res., 20, 4507-4513.
Pervushin, K., Riek, R., Wider, G. and Wüthrich, K. (1997) Proc. Natl. Acad. Sci. USA, 94, 12366-12371.
Pervushin, K., Riek, R., Wider, G. and Wüthrich, K. (1998) J. Am. Chem. Soc., 120, 6394-6400.
Ramachandran, R., Sich, C., Grune, M., Soskic, V. and Brown, L.R. (1996) J. Biomol. NMR, 7, 251-255.
Riek, R., Pervushin, K., Fernández, C., Kainosho, M. and Wüthrich, K. (2001) J. Am. Chem. Soc., 123, 658-664.
Salzmann, M., Pervushin, K., Wider, G., Senn, H. and Wüthrich, K. (1998) Proc. Natl. Acad. Sci. USA, 95, 13585-13590.
Salzmann, M., Pervushin, K., Wider, G., Senn, H. and Wüthrich, K. (2000) J. Am. Chem. Soc., 122, 7543-7548.
Sattler, M., Schleucher, J. and Griesinger, C. (1999) Prog. NMR Spectrosc., 34, 93-158.
Scott, L.G., Tolbert, T.J. and Williamson, J.R. (2000) Methods Enzymol., 317, 18-38.
Simorre, J.-P., Zimmermann, G.R., Müller, L. and Pardi, A. (1996) J. Biomol. NMR, 7, 153-156.
Sklenar, V., Dieckmann, T., Butcher, S.E. and Feigon, J. (1998) J. Magn. Reson., 130, 119-124.
Sklenar, V., Peterson, R.D., Rejante, M.R., Wang, E. and Feigon, J. (1992) J. Am. Chem. Soc., 115, 12181-12182.
Tate, S., Ono, A. and Kainosho, M. (1995) J. Magn. Reson., B106, 89-91.
Varani, G., Aboul-ela, F. and Allain, F.H.-T. (1996) Progr. NMR Spectrosc., 29, 51-127.
Wijmenga, S. and van Buuren, B.N.M. (1998) Progr. NMR Spectrosc., 32, 287-387.
Wüthrich, K. (1986) NMR of Proteins and Nucleic Acids, Wiley, New York, NY.
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Simon, B., Zanier, K. & Sattler, M. A TROSY relayed HCCH-COSY experiment for correlating adenine H2/H8 resonances in uniformly 13C-labeled RNA molecules. J Biomol NMR 20, 173–176 (2001). https://doi.org/10.1023/A:1011214914452
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DOI: https://doi.org/10.1023/A:1011214914452