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Sequence and Genetic Analyses of the 3′ Terminus and Integration Sites of the RIII/Sa Mouse Mammary Tumor (MMTV) Exogenous Provirus

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Abstract

The RIII/Sa mouse mammary tumor virus (MMTV) is one of several exogenous MMTV viruses transmitted to suckling mice through the milk. We report herein the nucleotide sequence of the exogenous RIII/Sa provirus from the central Eco RI site through to the end of the U5 region encoded by the 3′ LTR. We also provide a detailed sequence analysis of ten different 3′ terminal exogenous MMTV proviral integration sites within mammary tumor DNA obtained by the technique of genome walking. Using a combination of Southern blotting with 3′ end probes and PCR utilizing a unique RIII/Sa specific 3′ primer, we confirm that the RIII/Sa provirus integrates multiple times in mouse mammary tumors but that little or no integration occurs in various normal tissues. Southern blotting analyses with 3′ end probes also indicate that RIII/Sa mice contain two additional endogenous MMTV loci, mtv-6 and mtv-17, not previously reported. The combined genetic analyses reported herein distinguish between exogenous proviral integrants and endogenous germline MMTV.

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References

  1. Haberman B.H., Lab Anim Sci 24, 935-937, 1974.

    PubMed  Google Scholar 

  2. Acha-Orbea H., Medicina (B Aires) 57 (Suppl. 2), 43-52, 1997.

    Google Scholar 

  3. Callahan R. and Smith G.H., Oncogene 19, 992-1001, 2000.

    PubMed  Google Scholar 

  4. Grimm S.L. and Nordeen S.K., J Virol 72, 9428-9435, 1998.

    PubMed  Google Scholar 

  5. Sarkar N.H., Virology 212, 490-499, 1995.

    PubMed  Google Scholar 

  6. van Leeuwen F. and Nusse R., Semin Cancer Biol 6, 127-133, 1995.

    PubMed  Google Scholar 

  7. Payvar F., DeFranco D., Firestone G.L., Edgar B., Wrange O., Okret S., Gustafsson J.A., and Yamamoto K.R., Cell 35, 381-392, 1983.

    PubMed  Google Scholar 

  8. Bramblett D., Hsu C.L., Lozano M., Earnest K., Fabritius C., and Dudley J., J Virol 69, 7868-7876, 1995.

    PubMed  Google Scholar 

  9. Kim M.H. and Peterson D.O., J Virol 69, 4717-4726, 1995.

    PubMed  Google Scholar 

  10. Qin W., Golovkina T.V., Peng T., Nepomnaschy I., Buggiano V., Piazzon I., and Ross S.R., J Virol 73, 368-376, 1999.

    PubMed  Google Scholar 

  11. Golovkina T.V., Piazzon I., Nepomnaschy I., Buggiano V., de Olano Vela M., and Ross S.R., J Virol 71, 3895-3903, 1997.

    PubMed  Google Scholar 

  12. Nepomnaschy I., Buggiano V., Goldman A., Deroche A., Bekinschtein P., Berguer P., and Piazzon I., Medicina (B Aires) 57, 327-331, 1997.

    Google Scholar 

  13. Scherer M.T., Ignatowicz L., Winslow G.M., Kappler J.W., and Marrack P., Annu Rev Cell Biol 9, 101-128, 1993.

    PubMed  Google Scholar 

  14. Fasel N., Buetti E., Firzlaff J., Pearson K., and Diggelmann H., Nucleic Acids Res 11, 6943-6955, 1983.

    PubMed  Google Scholar 

  15. Luther S., Shakhov A.N., Xenarios I., Haga S., Imai S., and Acha-Orbea H., Eur J Immunol 24, 1757-1764, 1994.

    PubMed  Google Scholar 

  16. Majors J.E. and Varmus H.E., J Virol 47, 495-504, 1983.

    PubMed  Google Scholar 

  17. Moore R., Dixon M., Smith R., Peters G., and Dickson C., J Virol 61, 480-490, 1987.

    PubMed  Google Scholar 

  18. Kozak C., Peters G., Pauley R., Morris V., Michalides R., Dudley J., Green M., Davisson M., Prakash O., Vaidya A., Hilgers J., Verstraeten A., Hynes N., Diggleman H., Peterson D., Cohen J.C., Sarkar N., Nusse R., Varmus H., and Callahan R., J Virol 61, 1651-1654, 1987.

    PubMed  Google Scholar 

  19. Tomonari K., Fairchild S., and Rosenwasser O.A., Immunol Rev 131, 131-168, 1993.

    PubMed  Google Scholar 

  20. Peters G., Placzek M., Brookes S., Kozak C., Smith R., and Dickson C., J Virol 59, 535-544, 1986.

    PubMed  Google Scholar 

  21. Barnett A., Mustafa F., Wrona T.J., Lozano M., and Dudley J.P., J Virol 73, 6634-6645, 1999.

    PubMed  Google Scholar 

  22. Brookes S., Placzek M., Moore R., Dixon M., Dickson C., and Peters G., Nucleic Acids Res 14, 8231-8245, 1986.

    PubMed  Google Scholar 

  23. Majors J.E. and Varmus H.E., Nature 289, 253-258, 1981.

    PubMed  Google Scholar 

  24. Etkind P.R., Szabo P., and Sarkar N.H., J Virol 41, 855-867, 1982.

    PubMed  Google Scholar 

  25. Gunzburg W.H., Heinemann F., Wintersperger S., Miethke T., Wagner H., Erfle V., and Salmons B., Nature 364, 154-158, 1993.

    PubMed  Google Scholar 

  26. Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J., J Mol Biol 215, 403-410, 1990.

    Article  PubMed  Google Scholar 

  27. Peterson D.O., Kriz K.G., Marich J.E., and Toohey M.G., J Virol 54, 525-531, 1985.

    PubMed  Google Scholar 

  28. Cho K., Ferrick D.A., and Morris D.W., Virology 206, 395-402, 1995.

    PubMed  Google Scholar 

  29. Brandt-Carlson C., Butel J.S., and Wheeler D., Virology 193, 171-185, 1993.

    PubMed  Google Scholar 

  30. Peters G., Lee A.E., and Dickson C., Nature 320, 628-631, 1986.

    PubMed  Google Scholar 

  31. Lepage P., Devault A., and Gros P., Mol Cell Biol 13, 7380-7392, 1993.

    PubMed  Google Scholar 

  32. Dudley J.P., J Virol 62, 472-478, 1988.

    PubMed  Google Scholar 

  33. Wang J.Y., Ledley F., Goff S., Lee R., Groner Y., and Baltimore D., Cell 36, 349-356, 1984.

    PubMed  Google Scholar 

  34. Sawyers C.L., McLaughlin J., Goga A., Havlik M., and Witte O., Cell 77, 121-131, 1994.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. David F. Hickok Memorial Cancer Research Laboratory, Abbott Northwestern Hospital, 800 E. 28th St, Minneapolis, MN, 55407

    Pamela Popken-Harris & Lara Pliml

  2. The University of Minnesota Cancer Center, Minneapolis, MN, 55455

    Pamela Popken-Harris

  3. University of Minnesota Cancer Center, Minneapolis, MN 55455, 800 E. 28th St. Minneapolis, MN 55407; The

    Lester Harris

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  1. Pamela Popken-Harris
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  2. Lara Pliml
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  3. Lester Harris
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Popken-Harris, P., Pliml, L. & Harris, L. Sequence and Genetic Analyses of the 3′ Terminus and Integration Sites of the RIII/Sa Mouse Mammary Tumor (MMTV) Exogenous Provirus. Virus Genes 23, 35–43 (2001). https://doi.org/10.1023/A:1011175112113

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  • DOI: https://doi.org/10.1023/A:1011175112113