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Characterisation of head kidney and liver microsomal 17-hydroxyprogesterone 21-hydroxylase activity in rainbow trout (Oncorhynchus mykiss) and the lack of regulatory effects of ACTH

Fish Physiology and Biochemistry volume 24, pages 1–8 (2001)Cite this article

Abstract

The formation of 11-deoxycortisol from 17-hydroxyprogesterone was studied in rainbow trout (Oncorhynchus mykiss) head kidney and liver microsomes. The K m for head kidney microsomal 17-hydroxyprogesterone 21-hydroxylase measured in pooled microsomes from juvenile rainbow trout was 3.75 μM, and the V max was 41.4 pmol/(mg protein × min). The K m for liver microsomal 17-hydroxyprogesterone 21-hydroxylase was 31.0 μM, and the V max was 51.0 pmol/(mg protein × min). The intrinsic clearance values suggest that liver, in addition to head kidney, could significantly contribute to the formation of 11-deoxycortisol in rainbow trout. Progesterone inhibited both the head kidney and the liver microsomal 17-hydroxyprogesterone 21-hydroxylase activity competitively. The K i for progesterone in head kidney was 1.8 μM and in liver 144 μM. The different K m and K i values for microsomal 21-hydroxylation suggest that 21-hydroxylation of 17-hydroxyprogesterone in head kidney and liver is catalysed by different enzyme systems. Treatment of rainbow trout by adrenocorticotrophic hormone (ACTH) for 1.5 or 3 h (single injections) or three weeks (injections every other day) did not affect head kidney or liver17-hydroxyprogesterone 21-hydroxylase activity. ACTH may thus not regulate this enzyme activity. The increase in plasma cortisol levels 1.5 and 3 h after ACTH injection but not following repeated injections suggests that the hormone regulates the initial secretion of cortisol, but does not have a long-term regulatory effect upon cortisol production in rainbow trout.

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Authors and Affiliations

  1. Department of Zoophysiology, University of Göteborg, Box 463, SE 405 30, Göteborg, Sweden

    S. Blom & L. Förlin

  2. AstraZeneca R&D, S-431 83, Mölndal, Sweden

    T.B. Andersson

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  1. S. Blom
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  2. L. Förlin
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  3. T.B. Andersson
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Blom, S., Förlin, L. & Andersson, T. Characterisation of head kidney and liver microsomal 17-hydroxyprogesterone 21-hydroxylase activity in rainbow trout (Oncorhynchus mykiss) and the lack of regulatory effects of ACTH. Fish Physiology and Biochemistry 24, 1–8 (2001). https://doi.org/10.1023/A:1011148604039

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  • DOI: https://doi.org/10.1023/A:1011148604039

  • adrenocorticotrophic hormone
  • cortisol
  • corticosteroid production
  • enzyme kinetics
  • 21-hydroxylation

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