Abstract
The surface of the protozoan Trypanosoma cruzi, the etiologic agent of Chagas' disease, is covered by a dense glycolipid layer, composed mainly by a structurally related family of glycoinositolphospholipids (GIPLs). In the present study we evaluated the in vivo effects of the GIPL on B cell function and immunoglobulin (Ig) secretion. We observed that GIPL injection led to a sustained increase in circulating IgM levels. B cells from GIPL injected mice showed higher response when activated in vitro with either LPS or dextran-conjugated anti-IgD antibodies or purified cytokines. GIPL purified from T. cruzi also showed an adjuvant effect, since this glycophospholipid boosted a polysaccharide-(TNP-Ficoll) induced IgG response. Taken together, our data indicate that T. cruzi-derived GIPL could be at least partially responsible for the remarkable B cell activation observed during T. cruzi acute infection in vivo.
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Bilate, A.M., Previato, J.O., Mendonça-Previato, L. et al. Glycoinositolphospholipids from Trypanosoma cruzi induce B cell hyper-responsiveness in vivo. Glycoconj J 17, 727–734 (2000). https://doi.org/10.1023/A:1011082925179
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DOI: https://doi.org/10.1023/A:1011082925179