Abstract
To date, vertebrate DNA has been found methylated at the 5′ position of cytosine exclusively in dinucleotide CpG or CpNpG stretches. On the the other hand, we determined that cytosine was methylated unusually in dinucleotide GpC at 5′-GGCC-3′ sequences in the teleost Sparus aurata EcoRI satellite DNA family. This finding is the first example of methylated GpC sequences in the eukaryotic genomes. At this regard, we have examined the relative methylation levels at this site of the highly repetitive EcoRI satellite DNA family from Sparus aurata different tissues. The EcoRI repeat was remarkably more methylated in male germ cells but hypomethylated in female germ cells at the Hae III restriction site ( GpC). The novel modification and the differential methylation pattern suggest that EcoRI satellite could have a structural and/or functional role at the centromeres of Sparus aurata.
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Katsiotis A, Hagidimitriou M, Douka A & Hatzopoulos P (1998) Genome 4: 527–534.
Pintor-Toto JA (1987) Biochem. Biophys. Res. Commun. 3: 1082–1087.
Qu GZ, Grundy PE, Narayan A & Ehriich M (1999) Cancer Genet. Cytogenet. 109: 34–39.
Ji W, Hernandez R, Zhang XY, Qu GZ, Frad YA, Varela M & Ehrlich M (1997) Mutat. Res. 379: 33–41.
Miniou P, Bourc'his D, Molina Gomes D, Jeanpierre M & Viegas-Pequignot E (1997) Cytogenet. Cell. Genet. 77: 303–313.
Narayan A, Ji W, Zhang XY, Marrogi A, Graff JR, Baylin SB & Ehrlich M (1998) Int. J. Cancer 77: 833–838.
Schuffenhauer S, Bartsch O, Stumm M, Buchholz T, Petropoulou T, Kraft S, Belohradsky B, Hinkel GK, Meitinger T & Wegner RD (1995) Hum. Genet. 96: 562–571.
Dante R, Dante-Paire J, Rigal D & Roizes G (1992) Anti-cancer Res. 12: 55–63.
Hendrich B & Birds A (1998) Mol. Cell. Biol. 18: 6538–6547.
Mitchell AR, Jeppesen P, Nicol L, Morrison H & Kipling D (1996) J. Cell. Sci. 109: 2199–2206.
Weitzel JM, Buhrmeister H & Stratling WH (1997) Mol. Cell. Biol. 17: 5656–5666.
Davie JR & Delcuve GP (1988) Biochem. Cell. Biol. 66: 256–261.
Garrido-Ramos MA, Jamilena M, Lozano R, Ruiz Rejon C & Ruiz Rejon M (1995) Cytogenet. Cell Genet. 71: 345–351.
Garrido-Ramos MA, Jamilena M, Lozano R, Ruiz Rejon C & Ruiz Rejon M (1994) Cytogenet. Cell. Genet. 4: 233–237.
Rahe B, Erickson RP & Quinto M (1983) Nucl. Acids Res. 11: 7947–7959.
Sambrook J, Fritsh EF & Maniatis T (1982) Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Herman JG, Graff JR, Myohanen, Nelkin BD & Baylin SB (1996) PNAS. 93: 9821–9826.
Kato M (1996) Mol. Biol. Rep. 23: 123–127.
Sanford J, Forrester L, Chapman VM & Rossant J (1987) Genes Dev. 1: 1039–1046.
Gama-Sosa MA, Wang YH, Kno KC and Gehrke CW & Ehrlich M (1983) Nucl. Acids Res. 11: 3087–3095.
Howlett SK & Reik W (1991) Development 113: 119–127.
Rubin CM, VandeVoort CA, Teplitz RL & Schmid CW(1994) Nucl. Acids Res. 22: 5121–5127.
Pontecorvo G, De Felice B & Carfagna M (1998) FEBS Lett. 438: 77–82.
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Pontecorvo, G., De Felice, B. & Carfagna, M. Novel methylation at GpC dinucleotide in the fish Sparus aurata genome. Mol Biol Rep 27, 225–230 (2000). https://doi.org/10.1023/A:1011003928698
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DOI: https://doi.org/10.1023/A:1011003928698