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Filamentous fungus Aspergillus oryzae has two types of α-1,2-mannosidases, one of which is a microsomal enzyme that removes a single mannose residue from Man9GlcNAc2

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Abstract

α-Mannosidase activities towards high-mannose oligosaccharides were examined with a detergent-solubilized microsomal preparation from a filamentous fungus, Aspergillus oryzae. In the enzymatic reaction, the pyridylaminated substrate Man9GlcNAc2-PA was trimmed to Man8GlcNAc2-PA which lacked one α-1,2-mannose residue at the nonreducing terminus of the middle branch (Man8B isomer), and this mannooligosaccharide remained predominant through the overall reaction. Trimming was optimal at pH 7.0 in PIPES buffer in the presence of calcium ion and kifunensine was inhibitory with IC50 below 0.1[emsp4 ]μM. These results suggest that the activity is the same type as was previously observed with human and yeast endoplasmic reticulum (ER) α-mannosidases. Considering these results together with previous data on a fungal α-1,2-mannosidase that trimmed Man9GlcNAc2 to Man5GlcNAc2 (Ichishima, E., et al. (1999) bit>Biochem J, 339: 589–597), the filamentous fungi appear to have two types of α-1,2-mannosidases, each of which acts differently on N-linked mannooligosaccharides.

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References

  1. Herscovics A, Biochim Biophys Acta 1426, 275–285 (1999).

    Google Scholar 

  2. Herscovics A, Biochim Biophys Acta 1473, 96–107 (1999).

    Google Scholar 

  3. Tremblay LO, Herscovics A, Glycobiology 9, 1073–1078 (1999).

    Google Scholar 

  4. Gonzalez DS, Karaveg K, Vandersall-Nairn AS, Lal A, Moremen KW, J Biol Chem 274, 21375–21386 (1999).

    Google Scholar 

  5. Tremblay LO, Herscovics A, J Biol Chem 275, 31655–31660 (2000).

    Google Scholar 

  6. Becker B, Haggarty A, Romero PA, Poon T, Herscovics A, Eur J Cell Biol 79, 986–992 (2000).

    Google Scholar 

  7. Igdoura SA, Herscovics A, Lal A, Moremen KW, Morales CR, Hermo L, Eur J Cell Biol 78, 441–452 (1999).

    Google Scholar 

  8. Lal A, Pang P, Kalelkar S, Romero PA, Herscovics A, Moremen KW, Glycobiology 8, 981–995 (1998).

    Google Scholar 

  9. Ichishima E, Arai M, Shigematsu Y, Kumagai H, Sumida-Tanaka R, Biochim Biophys Acta 658, 45–53 (1981).

    Google Scholar 

  10. Yoshida T, Inoue T, Ichishima E, Biochem J 290, 349–54 (1993).

    Google Scholar 

  11. Yoshida T, Ichishima E, Biochim Biophys Acta 1263, 159–162 (1995).

    Google Scholar 

  12. Inoue T, Yoshida T, Ichishima E, Biochim Biophys Acta 1253, 141–145 (1995).

    Google Scholar 

  13. Yoshida T, Nakajima T, Ichishima E, Biosci Biotechnol Biochem 62, 309–315 (1998).

    Google Scholar 

  14. Ichishima E, Taya N, Ikeguchi M, Chiba Y, Nakamura M, Kawabata C, Inoue T, Takahashi K, Minetoki T, Ozeki K, Kumagai C, Gomi K, Yoshida T, Nakajima T, Biochem J 339, 589–597 (1999).

    Google Scholar 

  15. Eades CJ, Hintz WE, Gene 255, 25–34 (2000).

    Google Scholar 

  16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ, J Biol Chem 193, 265–275 (1951).

    Google Scholar 

  17. Elbein AD, Tropea JE, Mitchell M, Kaushal GP, J Biol Chem 265, 15599–15605 (1990).

    Google Scholar 

  18. Weng S, Spiro RG, J Biol Chem 268, 25656–25663 (1993).

    Google Scholar 

Download references

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Authors and Affiliations

  1. Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo, Hirosaki, Aomori, 036-8561, Japan

    Takashi Yoshida & Yoshihiro Asada

  2. Faculty of Education, Hirosaki University, 3 Bunkyo, Hirosaki, Aomori, 036-8561, Japan

    Yoji Kato

  3. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiya, Aoba, Sendai, 981-8555, Japan

    Tasuku Nakajima

Authors
  1. Takashi Yoshida
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  2. Yoji Kato
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  3. Yoshihiro Asada
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  4. Tasuku Nakajima
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Yoshida, T., Kato, Y., Asada, Y. et al. Filamentous fungus Aspergillus oryzae has two types of α-1,2-mannosidases, one of which is a microsomal enzyme that removes a single mannose residue from Man9GlcNAc2. Glycoconj J 17, 745–748 (2000). https://doi.org/10.1023/A:1010984608855

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  • DOI: https://doi.org/10.1023/A:1010984608855

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