, Volume 151, Issue 1, pp 29–33 | Cite as

Isolation of aflatoxigenic strains of Aspergillus and detection of aflatoxin B1 from feeds in India

  • T.K. Dutta
  • P. Das


In a preliminary study, 256 feed samples collected from different parts of Northern India were examined for the presence of aflatoxigenic strains of Aspergillus flavus/parasiticus and for detection of Aflatoxin B1 (AFB1). Out of 198 A. flavus and 15 A. parasiticus strains isolated, 76% and 86% respectively, were found to be toxigenic. Aflatoxin B1 content of these feeds, as estimated by thin layer chromatography (TLC) and enzyme linked immunosorbent assay (ELISA) were very high (average 0.412 ± 0.154 ppm) in comparison to the permissible Indian regulation level (0.03 ppm). Seasonal variation of incidence and level of toxin in feed was recorded and it was high during monsoon/post monsoon period.

aflatoxin B1 Aspergillus flavus/parasiticus feed India 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • T.K. Dutta
    • 1
  • P. Das
    • 1
  1. 1.Indian Veterinary Research InstituteIndia

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