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Plasma retinol and tocopherol levels in Greek elderly population from an urban and a rural area: Associations with the dietary habits

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Abstract

Data on plasma concentrations of retinol and α-tocopherol in elderly (over 65 years) living in the Mediterranean countries are sparse. In the current study, plasma retinol and α-tocopherol concentrations were determined in 200 healthy subjects (82 men and 112 women) residing in an urban and in a rural area in South Western Greece. High prevalence of low plasma retinol concentration was observed for both sexes in each area. The 16.7% of males in urban and 25.4% in rural area had plasma retinol levels below 0.3 mg/l. The corresponding values for female subjects were 26% and 20.3%. Plasma α-tocopherol was found to be within normal range in the rural area (only one male had plasma α-tocopherol below 5 mg/l) while in the urban area 68.3% of men and 62% of women had plasma α-tocopherol values below 5 mg/l. Differences in dietary habits between subjects living in the rural and in the urban area were revealed through a weekly food frequency questionnaire. This could partly explain the difference in α-tocopherol concentrations between the two areas. The results of this study revealed high risk of vitamin A and E deficiency and suggests that the health care system in Greece should pay more attention on the social and health status of its elderly population.

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References

  1. Harrman D. Prolongation of the normal lifespan and inhibition of spontaneous cancer by antioxidants. J Gerontol 16 1961; 16: 247–254.

    Google Scholar 

  2. Gey KF, Brubacher GB, Stahelin HB. Plasma levels of antioxidant vitamins in relation to ischemic heart disease. Am J Clin Nutr 1987; 45: 1368–1377.

    Google Scholar 

  3. Gey KF, Puska P, Jordan P, Moser UK. Inverse correlation between plasma vitamin E and mortality from ischemic heart disease in cross-cultural epidemiology. Am J Clin Nutr 1991; 53: S326-S334.

    Google Scholar 

  4. Rimm EB, Stampfer MJ, Ascherio A, Giovannucci E, Colditz GA, Willet WC. Vitamin E consumption and the risk of coronary heart disease in men. N Engl J Med 1993; 328: 1450–1456.

    Google Scholar 

  5. Chen J, Geissler C, Parpia B, Li J, Campell TC. Antioxidant status and cancer mortality in China. Int J Epidemiol 1992; 21: 625–635.

    Google Scholar 

  6. Meydani M. Vitamin E requirement in relation to dietary fish oil and oxidative stress in elderly. EXS 1992; 62: 411–418.

    Google Scholar 

  7. Brady WE, Marles-Perlman JA, Bower P, Stacewicz-Sapumtzakis M. Human serum carotenoid concentrations are related to physiologic and lifestyle factors. J Nutr 1996; 126: 129–137.

    Google Scholar 

  8. Olmedilla B, Granado F, Blanco I, Rojas-Hidalgo E. Seasonal and sex-related variations in six serum carotenoids, retinol and α-tocopherol in older adults. Am J Epidemiol 1988; 127: 114–123.

    Google Scholar 

  9. Euronut SENECA investigators. Nutritional status: Blood vitamins A, E, B6, B12, folic acid and carotene. Eur J Clin Nutr 1991; 45: 63–82.

    Google Scholar 

  10. Porrini M, Simonetti P, Ciappellano S, Testolin G. Vitamin A, E and C nutriture of elderly people in North Italy. Int J Vit Nutr Res 1987; 57: 349–355.

    Google Scholar 

  11. Miller KW, Yang CS. An isocratic high-performance liquid chromatography method for the simultaneous analysis of plasma retinol, α-tocopherol and various carotenoids. Anal Biochem 1985; 145: 21–26.

    Google Scholar 

  12. Kitamura Y, Tanaka K, Kiyohara C, et al. Relationship of alcohol use and dietary habits with serum carotenoids, retinol and α-tocopherol among Japanese smokers. Int J Epidemiol 1997; 26: 307–314.

    Google Scholar 

  13. Romieu I, Stampfer MJ, Stryker WS, et al. Food predictors of plasma b-carotene and a-tocopherol: Validation of a food frequency questionnaire. Am J Epidemiol 1990; 131: 864–876.

    Google Scholar 

  14. Azais-Braesco V, Moriniere C, Guesne B, et al. Vitamin A status in the institutionalized elderly. Critical analysis of four evaluation criteria: Dietary vitamin A intake, serum retinol, relative dose-response test (RDR) and impression cytology with transfer (ICT). Int J Vit Nutr Res 1995; 65: 151–161.

    Google Scholar 

  15. Marks J. The Vitamins: Their Role in Medical Practice. NY: MTP Press, 1985, pp 111–112.

    Google Scholar 

  16. Bendich A, Langseth L. Safety of Vitamin A. Am J Clin Nutr 1989; 49: 358–371.

    Google Scholar 

  17. Panemangalore M, Lee JC. Evaluation of the indices of retinol and a-tocopherol status in free living elderly. J Gerontol 1992; 47: B98-B104.

    Google Scholar 

  18. Herberth B, Chavance M, Musse N, Mejean L. Dietary intake and other determinants of blood vitamins in an elderly population. Eur J Clin Nutr 1989; 43: 175–186.

    Google Scholar 

  19. Heseker H, Schneider R, Moch KJ, Kohlmeier M, Kubler W. Vitamins status of adults in the Federal Republic of Germany. Vera Schriftenreihe-Band IV. Niederkleen, Germany. 1992 Wissenschaflicher Fachverlag Dr Fleck.

  20. Hallfrisch J, Muller D, Singh V. Vitamin A and E intakes and plasma concentrations of retinol, b-carotene, and a-tocopherol in men and women of the Baltimore longitudinal study of aging. Am J Clin Nutr 1994; 60: 176–182.

    Google Scholar 

  21. Pilch SM. Analysis of vitamin A data from the Health and Examination Surveys. J Nutr 1987; 117: 636–640.

    Google Scholar 

  22. Garry PJ, Hunt WC, Brandrofchack JL, Vanderjagt D, Goodwin JS. Vitamin A intake and plasma retinol levels in healthy elderly men and women. Am J Clin Nutr 1987; 46: 989–994.

    Google Scholar 

  23. Jacques PF, Sulsky SI, Sadowski JA, Phillips JCC, Rush D, Willett WC. Comparison of micronutrient intake measured by a dietary questionnaire and biochemical indicators of micronutrient status. Am J Clin Nutr 1993; 57: 182–189.

    Google Scholar 

  24. Looker AC, Johnson CL, Underwood BA. Serum retinol levels of persons aged 4–74 years from three Hispanic groups. Am J Clin Nutr 1988; 48: 1490–1496.

    Google Scholar 

  25. Krasinski SD, Rusell RM, Otradovec CL. Relationship of vitamin A and E intake to fasting plasma retinol, retinol binding protein, retinyl esters, carotene, α-tocopherol and cholesterol among elderly people and young adults: Increased plasma retinyl esters among vitamin A supplement users. Am J Clin Nutr 1989; 49: 112–120.

    Google Scholar 

  26. Harrell I, Cervone N. Vitamin status of older women. Am J Clin Nutr 1997; 30: 431–440.

    Google Scholar 

  27. Vogel S, Contois J, Tucker K, Wilson P, Schaefer E, Keefe-Lammi CJ. Plasma retinol and plasma and lipoprotein tocopherol and carotenoid concentrations in health elderly participants of the Framingham Heart Study. Am J Clin Nutr 1997; 66: 950–958.

    Google Scholar 

  28. Monget AL, Galan P, Preziosi P, et al. Micronutrients status in elderly population. Int J Vit Nutr Res 1996; 66: 71–76.

    Google Scholar 

  29. Ito Y, Ochiai J, Sasaki R, et al. Serum concentrations of carotenoids, retinol and a-tocopherol in healthy persons determined by HPLC. Clinica Chimica Acta 1990; 194: 131–144.

    Google Scholar 

  30. Comstock GW, Menkes MS, Schober SE, Vuilleumier JP, Helsing KJ. Serum levels of retinol, b-carotene and α-tocopherol in older adults. Am J Epidemiol 1987; 127: 114–123.

    Google Scholar 

  31. Trichopoulou A, Lagiou P, Trichopoulos D. Traditional Greek diet and coronary heart disease. J Cardiovascular Risk 1994; 1: 9–15.

    Google Scholar 

  32. Thompson MP, Mongan PF, Miller MD, Creten D. Comparison of dietary intake of urban and rural elderly patients in family practice centers. Southern Med J 1987; 80: 1216–1219.

    Google Scholar 

  33. Vandewoude MF, Vandewoude MG. Vitamin E status in normal population: The influence of age. J Am Coll Nutr 1987; 6: 307–311.

    Google Scholar 

  34. Knekt P, Aromaa A, Maatela J. Serum vitamin E and risk of cancer among Finnish men during a 10-year follow-up. Am J Epidemiol 1988; 127: 28–41.

    Google Scholar 

  35. Hooper P, Hooper E, Hunt W, Garry P, Goodwin J. Vitamins, lipids and lipoproteins in a health elderly population. Int J Vit Nutr Res 1983; 53: 412–419.

    Google Scholar 

  36. Bates CJ, Burr MK, Leger AS. Vitamin C, high density lipoproteins and heart disease in elderly subjects. Age Ageing 1979; 8: 177–182.

    Google Scholar 

  37. Burr ML, Bates CJ, Sweetnam PM, Barasi ME. Plasma ascorbate and HDL-cholesterol in women. Hum Nutr Clin Nutr 1982; 36C: 399–400.

    Google Scholar 

  38. Ginter E, Kubec FJ, Vozar J, Boker P. Natural hypocholesterolemic agent: Pectin plus ascorbic acid. Int J Vitam Nutr Res 1979; 49: 406–412.

    Google Scholar 

  39. Howard DR, Rundell CA, Batsakis JG. Vitamin E does not modify HDL-cholesterol. Am J Clin Pathol 1982; 77: 86–89.

    Google Scholar 

  40. Johnson GE, Obenshain SS. Nonresponsiveness of serum high-density lipoprotein-cholesterol to high dose ascorbic acid administration in normal men. Am J Clin Nutr 1981; 34: 2088–2091.

    Google Scholar 

  41. Schwartz PL, Rutherford IM. The effect of tocopherol on high-density lipoprotein cholesterol. Am J Clin Pathol 1981; 76: 843–845.

    Google Scholar 

  42. Peterson VE, Crapo PA, Weininger J, Ginsberg H, Olefsky L. Quantification of plasma cholesterol and triglyceride levels in hypercholesterolemic subjects receiving ascorbic acid supplements. Am J Clin Nutr 1975; 28: 584–587.

    Google Scholar 

  43. Kaplan LA, Stein EA, Willett WC, Stampfer MJ, Stryker WS. Reference ranges of retinol, tocopherols, lycopene and a-and b-carotene in plasma by simultaneous HPLC analysis. Clin Physiol Biochem 1987; 5: 297–304.

    Google Scholar 

  44. Vuilleumier JP, Keller HE, Gysel D. Clinical chemical methods for the routine assessment of the vitamin status in human populations. Part I: The fat soluble vitamins A and E and β-carotene. Int J Vit Nutr Res 1983; 53: 266–272.

    Google Scholar 

  45. Bowman BB, Rosenberg IH. Assessment of the nutritional status of the elderly. Am J Clin Nutr 1982; 35: 1142–1151.

    Google Scholar 

  46. Succari M, Garric B, Ponteziere C, Miocque M, Cals MJ. Influence of sex and age on vitamin A and E status. Age Ageing 1991; 20: 413–416.

    Google Scholar 

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Leotsinidis, M., Alexopoulos, A., Schinas, V. et al. Plasma retinol and tocopherol levels in Greek elderly population from an urban and a rural area: Associations with the dietary habits. Eur J Epidemiol 16, 1009–1016 (2000). https://doi.org/10.1023/A:1010895227352

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