Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture

Abstract

An efficient system for the in vitro plant and shootregeneration of Lilium longiflorum was developed andaccomplished using transverse thin cell layers (tTCL) of young stems.tTCLs were cut transversely along young stems from which the shoot-tipshad been removed. Sections were measured accurately using a graded gridand were cut in 4 mm × 4 mm × 1 mm cubes, eliminatingepidermal tissue, and were cultured on one-half MS medium containing 8 gl⁻¹ agar, different sucrose concentrations (10, 20, 30 or 40g l⁻¹), and with or without 1 mg l⁻¹ activatedcharcoal (AC). Plants formed on the surface of tTCLs within 60 days onone-half MS medium containing 8 g l⁻¹ agar and 20 gl⁻¹ sucrose. Sections of 1 mm taken just below the apicalarea developed buds within 15 days, whereas the sections closer to thebase required about 45 days. Shoot regeneration was enhanced whensucrose concentration was used at 30 or 40 g l⁻¹ after 60days of culture. No root formation occurred. Both shooting and rootingoccurred when sucrose was used at 20 g l⁻¹. The plantletswere transferred to soil and grew well under greenhouseconditions.