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Micropropagation of Centella asiatica (L.), a valuable medicinal herb

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Abstract

A protocol is described for rapid and large-scale in vitro clonal propagation of the valuable medicinal herb Centella asiatica (L.) by enhanced axillary bud proliferation in nodal segments isolated from mature plants. Although bud break was dependent on BA supply, the synergistic combination of 22.2 μM BA and 2.68 μM NAA induced the optimum frequency (91%) of shoot formation as well as shoot number (4 to 5 shoots per node). Subculturing of nodal segments harvested from the in vitro derived axenic shoots on the multiplication medium enabled continuous production of healthy shoots with similar frequency. MS medium supplemented with 6.7 μM BA and 2.88 μM IAA was found most suitable for shoot elongation. Rooting was highest (90%) on full-strength MS medium containing 2.46 μM IBA. Micropropagated plants established in garden soil were uniform and identical to the donor plant with respect to growth characteristics. This micropropagation procedure could be useful for raising a stock of genetically homogenous plant material for field cultivation.

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Authors and Affiliations

  1. Department of Botany, Government Arvind College, Kirandul, Bastar-, 494556, India; requests for offprints; Tel: +91-07857-55625; Fax: +91-07857-55122)

    Kavindra Nath Tiwari

  2. Department of Botany, SBSPGI Bio-Medical Sciences and Research, Balawala, Dehradun, India

    Nilesh Chandra Sharma

  3. School of Biotechnology, Faculty of Sciences, Banaras Hindu University, Varanasi-, 221005, India

    Vaibhav Tiwari & Brahma Deo Singh

Authors
  1. Kavindra Nath Tiwari
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  2. Nilesh Chandra Sharma
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  3. Vaibhav Tiwari
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  4. Brahma Deo Singh
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Nath Tiwari, K., Chandra Sharma, N., Tiwari, V. et al. Micropropagation of Centella asiatica (L.), a valuable medicinal herb. Plant Cell, Tissue and Organ Culture 63, 179–185 (2000). https://doi.org/10.1023/A:1010690603095

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  • DOI: https://doi.org/10.1023/A:1010690603095

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