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Physicochemical Factors Affecting the Sensitivity of Ceriodaphnia dubia to Copper

Abstract

The effects of physicochemical conditions, such as pH, water hardness, flow rates and natural organic substances on the sensitivity of Ceriodaphnia dubia to the toxic effects of copper were investigated using static bioassay cups and specially designed flow-through bioassay chambers. We found that C.dubia was very sensitive to pH changes and the total copper LC50 values of C. dubia neonates increased by 15-fold as the pH increased from pH 7 to 10. It was also observed that the LC50 values increased sharply upon increasing the water hardness value to 2.4 meq. In addition, increasing flow rates from zeroto 50 mL hr-1 also increased its sensitivity to copper, which was possibly due to hydrodynamic stress.The presence of natural organic substances (humic acid and dissolved organic matter) and suspended particles decreased thetoxic effect of copper. This significant decrease in the toxicity of copper in the presence of natural organic materialscan be explained by a reduction in the free ion concentration due to complexation. Furthermore, we observed that the kinetics of copper interactions with natural organic materials are a significant factor in the toxic effect of copper and that the acute LC50 values increased with increasing reaction time betweensolubilized copper and water-borne organics.

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References

  • Belanger, S. E. and Cherry, D. S.: 1990, ‘Interacting effects of pH acclimation, pH, and heavy metals on acute and chronic toxicity to Ceriodaphnia dubia (Cladocera)’, J. Crust. Biol. 10, 225–235.

    Google Scholar 

  • Borgmann, U.: 1983, ‘Metal Speciation and Toxicity of Free Metal Ions to Aquatic Biota’, in: J. O. Nriagu (ed.), Aquatic Toxicology, Wiley Interscience, New York, pp. 47–71.

    Google Scholar 

  • Cairns Jr., J., Health, A. G. and Parker, B. C.: 1975, ‘The effects of temperature upon the toxicity of chemicals to aquatic organisms’, Hydrobiol. 47, 135–171.

    Google Scholar 

  • Cusimano, R. F., Brakke, D. F. and Chapman, G. A.: 1986, 'Effects of pH on the toxicities of cadmium, copper, and zinc to steelhead trout (Salmo gairdneri), Can. J. Fish. Aquat. Sci 43, 1497–1503.

    Google Scholar 

  • Erickson, R. J., Benoit, D. A., Mattson, V. R., Nelson Jr., H. P. and Leonard, E. N.: 1996, ‘The effects of water chemistry on the toxicity of copper to fathead minnows’, Environ. Toxicol. Chem. 15, 181–193.

    Google Scholar 

  • Finney, D. J.: 1971, Probit Analysis, Cambridge University Press, New York.

    Google Scholar 

  • Gundersen, D. T. and Curtis, L. R.: 1995, ‘Acclimation to hard or soft water at weakly alkaline pH influences gill permeability and gill surface calcium binding in rainbow trout (Oncorhyncys mykiss)’, Can. J. Fish. Aquat. Sci. 52, 2583–2593.

    Google Scholar 

  • Kim, S. D., Ma, H., Allen, H. E. and Cha, D. K.: 1999, ‘Influence of dissolved organic matter on the toxicity of copper to Ceriodaphnia dubia: Effect of complexation kinetics’, Environ. Toxicol. Chem. 18, 2433–2437.

    Google Scholar 

  • Lauren, D. J. and McDonald, D. G.: 1986, ‘Influence of water hardness, pH, and alkalinity on the mechanisms of copper toxicity in juvenile rainbow trout, Salmo gairdneri’, Can. J. Fish. Aquat. Sci. 43, 1488–1496.

    Google Scholar 

  • Ma, H., Kim S. D., Cha, D. K., Allen, H. E.: 1999, ‘Effect of kinetics of complexation by humic acid on toxicity of copper to Ceriodaphnia dubia’, Environ. Toxicol. Chem. 18, 828–837.

    Google Scholar 

  • Mayer Jr., F. L. and Ellersieck, M. R.: 1988, ‘Experiences with single-species tests for acute toxicity effects in freshwater animals’, Ambio 17, 367–375.

    Google Scholar 

  • Miller, T. G. and MacKay, W. C.: 1980, ‘The effects of hardness, alkalinity and pH of test water on the toxicity of copper to rainbow trout (Salmo gairdneri)’, Water Res. 114, 129–133.

    Google Scholar 

  • Muller, B. and Sigg, L.: 1990, ‘Interaction of trace metals with natural particle surfaces: Comparison between adsorption experiments and field measurements’, Aquatic. Sci. 53, 75–92.

    Google Scholar 

  • Part, P., Svanberg, O. and Kiessling, A.: 1985, ‘The availability of cadmium to perfused rainbow trout gills in different water qualities’, Water Res. 19, 427–434.

    Google Scholar 

  • Penttinen, S., Kostamo, A. and Kukkonen, J. V. K.: 1998, ‘Combined effects of dissolved organic material and water hardness on toxicity of cadmium to Daphnia magna’, Environ. Toxicol. Chem. 17, 2498–2503.

    Google Scholar 

  • Peterson, H. G., Healey, F. P. and Wagemann, R.: 1984, ‘Metal toxicity to algae: A highly pH dependent phenomenon’, Can. J. Fish. Aquat. Sci. 41, 974–978.

    Google Scholar 

  • Rachlin, J. W. and Grosso, A.: 1991, ‘The effects of pH on the growth of Chlorella vulgaris and interactions with cadmium toxicity’, Arch. Environ. Contam. Toxicol. 20, 505–508.

    Google Scholar 

  • Serkiz, S.M. and Perdue, E.M.: 1990, ‘Isolation of dissolved organic matter from the Suwanee river using reverse osmosis’, Water Res. 24, 911–916.

    Google Scholar 

  • Sprague, J. B.: 1985, ‘Factors that Modify Toxicity’, in: G. M. Rand and S. R. Petrocelli (eds), Fundamentals of Aquatic Toxicology, Hemisphere, Washington, pp. 124–163.

    Google Scholar 

  • Stewart, A. J. and Konetsky, B. K.: 1998, ‘Longevity and reproduction of Ceriodaphnia dubia in receiving waters’, Environ. Toxicol. Chem. 17, 1165–1171.

    Google Scholar 

  • U.S. EPA: 1985, Ambient Water Quality Criteria for Copper – 1984, EPA 440/5–84–031,Washington, D.C.

  • U.S. EPA: 1993, Method for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms, EPA/600/4–90/027F, Washington, D.C.

  • Welsh, P. G., Skidmore, J. F., Spry, Dixon, D. G., Hodson, P. V., Hutchinson, N. J. and Hickie, B. E.: 1993, ‘Effect of pH and dissolved organic carbon on the toxicity of copper to larval fathead minnow (Pimephales promelas) in natural waters of low alkalinity’, Can. J. Fish. Aquat. Sci. 50, 1356–1362.

    Google Scholar 

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Correspondence to Sang Don Kim.

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Kim, S.D., Gu, M.B., Allen, H.E. et al. Physicochemical Factors Affecting the Sensitivity of Ceriodaphnia dubia to Copper. Environ Monit Assess 70, 105–116 (2001). https://doi.org/10.1023/A:1010689432130

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  • DOI: https://doi.org/10.1023/A:1010689432130

  • complexation
  • copper
  • natural organic substances
  • reaction time
  • toxicity