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Cytokine mRNA Levels and Lymphocyte Infiltration in Pancreatic Tissue During Experimental Chronic Pancreatitis Induced by Dibutyltin Dichloride

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Abstract

There is little information available regarding the role of inflammatory cells in the pathogenesis of chronic pancreatitis. Therefore, we analyzed the local cytokine profile and infiltrating lymphocytes in a rat model of chronic pancreatitis. Experimental pancreatitis was induced by a single intravenous application of dibultyltin dichloride (DBTC). During a time course of two months we observed the mRNA expression of cytokines using competitive RT-PCR. Lymphocytes were characterized by immunohistochemistry, FACS analysis, and the lymphocyte proliferation test. IL-1β, IL-6, IL-5, and IL-10 were immediately up-regulated in the acute phase of disease, while lymphocyte-restricted expression of IL-2, IL-2R, and IFN-γ was only found in the chronic course. Among the infiltrating lymphocytes, CD4+ cells dominated, but during the chronic process there was an increase of CD8+ cells, resulting in a reduced CD4/CD8 ratio. Mitogen-induced activation of isolated mesenteric lymph node cells increased during the chronic inflammation. Our results suggest that in experimental pancreatitis acute inflammatory reactions are followed by a T-lymphocyte-mediated process.

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Authors and Affiliations

  1. Department of Gastroenterology, University of Rostock, Rostock, Germany

    Gisela Sparmann, Stefan Behrend, Stefan Liebe & Jõrg Emmrich

  2. Institute of Pharmacology, University of Rostock, Rostock, Germany

    Jutta Merkord

  3. Department of Hematology and Oncology, University of Rostock, Rostock, Germany

    Hans-Dieter Kleine

  4. Institute of Medical Immunology, Medical Faculty (Charité), Humboldt University, Berlin, Germany

    Elmara Graser & Thomas Ritter

Authors
  1. Gisela Sparmann
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  2. Stefan Behrend
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  3. Jutta Merkord
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  4. Hans-Dieter Kleine
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  5. Elmara Graser
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  6. Thomas Ritter
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  7. Stefan Liebe
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  8. Jõrg Emmrich
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Sparmann, G., Behrend, S., Merkord, J. et al. Cytokine mRNA Levels and Lymphocyte Infiltration in Pancreatic Tissue During Experimental Chronic Pancreatitis Induced by Dibutyltin Dichloride. Dig Dis Sci 46, 1647–1656 (2001). https://doi.org/10.1023/A:1010689117772

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