Abstract
Salt tolerant (R100) and sensitive (S100) cell lines of Glycine max (L.) that differ in their ability to accumulate sodium (Na) and chloride (Cl) under 100 mM salt stress, were used to compare the contribution of carbohydrates in osmotic adjustment. Calliwere exposed to a 100 mM NaCl concentrations for 12 days followed by 16 days of relief from stress to determine the effect of salinity changes of sugar content in the two cell lines. The salt-tolerant and the salt-sensitive cell lines differed in the time at which, and the type of sugar, that increased during salt stress. However, recovery in sugar content parameters during relief from stress were similar in the two cell lines. The concentration of glucose and fructose increased at a rate closely corresponding to the increase in fresh weight, while the concentration of sucrose decreased to the control level coincident with relatively rapid growth.
Similar content being viewed by others
References
Ackerson RC (1981) Osmoregulation in cotton in response to water stress II. Leaf carbohydrate status in relation to osmotic adjustment. Plant Physiol 67: 489–493
Avigad G (1982) Sucrose and disaccharides. In: Lowus FA and Tanner W (eds) Encyclopedia of Plant Physiology. Springer-Verlag Berlin, pp 217–347
Bolarín MC, Santa-Cruz A, Cayuela E and Pérez-Alfocea F (1995) Short-term solute changes in leaves and roots of cultivated and wild tomato seedlings under salinity. J Plant Physiol 147: 463–468
Eaton FM and Ergle DR (1948) Carbohydrate accumulation in the cotton plant at low moisture levels. Plant Physiol 23: 169–187
Ehness R, Ecker M, Godt DE and Roitsch T (1997) Glucose and stress independently regulate source and sink metabolism and defense mechanisms via signal transduction pathways involving protein phosphorylation. Plant Cell 9: 1825–1841
Erdel L, Trivedi S, Takeda K and Matsumoto H (1990) Effects of osmotic and salt stresses on the accumulation of polyamines in leaf segments from wheat varieties differing in salt and drought tolerance. J Plant Physiol 137: 165-168
Kameli A and Lösel DM (1993) Carbohydrates and water status in wheat plants under water stress. New Phytol 125: 609–614
Kameli A and Lösel DM(1995) Contribution of carbohydrates and other solutes to osmotic adjustment in wheat leaves under water stress. J Plant Physiol 145: 363–366
Kameli A and Lösel DM (1996) Growth and sugar accumulation in Durum wheat plants under water stress. New Phytol 132: 57–62
Koch KE (1996) Carbohydrate-modulated gene expression in plants. Annu Rev Plant Physiol Plant Mol Biol 47: 509–540
Liu T and Van Staden J (2000) Selection and characterization of sodium chloride-tolerant callus of Glycine max (L.) Merr. cv. Acme. Plant Growth Regul (in press)
Liu T, Van Staden J and Cress WA (2000) Gene expression in soybean (Glycine max L.) roots exposed to low temperature: Isolation of cDNA clones. Plant Growth Regul 30: 247–251
McKersie BD and Leshem YY (1994) Stress and stress-coping in cultivated plants. Kluwer Academic Publishers London
Miller CO (1965) Evidence for the natural occurrence of zeatin and derivatives: compounds from maize which promote cell division. Proc Natl Acad Sci USA 54: 1052–1058
Morgan JM (1992) Osmotic components and properties associated with genotypic differences in osmoregulation in wheat. Aust J Plant Physiol 19: 67–76
Morris DA and Arthur ED (1984) Invertase activity in sinks undergoing cell expansion. Plant Growth Regul 2: 327–337
Perez-Alfocea F, Bolarin MC and Guerrier G (1995) Sucrose metabolism in NaCl-treated calli from Lycopersicon esculentum, L. pennellii and their interspecific hybrids. J Plant Physiol 145: 161–167
Roitsch T, Bittner M and Godt DE (1995) Induction of apoplastic invertase of Chenopodium rubrum by D-glucose and a glucose analog and tissue specific expression suggest a role in sink source regulation. Plant Physiol 108: 285–294
Sacher RF and Staples RC (1985) Inositol and sugars in adaptation of tomato to salt. Plant Physiol 77: 206–210
Salanoubat M and Belliard G (1989) The steady-state level of potato sucrose synthase mRNA is dependent on wounding, anaerobiosis and sucrose concentration. Gene 84: 181–185
Sheen J, Zhou L and Jang J (1999) Sugars as signaling molecules. Curr Opinion Plant Biol 2: 410–418
Tsukaya H, Ohshima T, Naito S, Chino M and Komeda Y (1991) Sugar-dependent expression of the CHS-A gene for chalcone synthase from petunia in transgenic Arabidopsis. Plant Physiol 97: 1414–1421
Visser RGF, Stolte A and Jacobsen E (1991) Expression of a chimeric granule-bound starch synthase-GUS gene in transgenic potato plants. Plant Mol Biol 17: 691–699
Wang Z, Quebedeaux B and Stutte GW (1995) Osmotic adjustment: effect of water stress on carbohydrates in leaves, stems, and roots of apple. Aus J Plant Physiol 22: 747–754
Zhang BL and Archbold DD (1993) Solute accumulation in leaves of a Fragaria chiloensis and a F. virginiana selection responds to water deficit stress. J Am Soc Hort Sci 118: 280–285
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liu, T., van Staden, J. Partitioning of carbohydrates in salt-sensitive and salt-tolerant soybean callus cultures under salinity stress and its subsequent relief. Plant Growth Regulation 33, 13–17 (2001). https://doi.org/10.1023/A:1010687711334
Issue Date:
DOI: https://doi.org/10.1023/A:1010687711334