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Electrogenic Properties of the Na+,K+-ATPase Probed by Presteady State and Relaxation Studies

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Abstract

Electrical measurements on planar lipid bilayers, patch/voltage clamp experiments, and spectroscopic investigations involving a potential sensitive dye are reviewed. These experiments were performed to analyze the kinetics of charge translocation of the Na+,K+-ATPase. High time resolution was achieved by applying caged ATP, voltage-jump, and stopped-flow techniques, respectively. Kinetic parameters and the electrogenicity of the relevant transitions in the Na+,K+-ATPase reaction cycle are discussed.

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Authors
  1. Ernst Bamberg
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  2. Ron J. Clarke
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  3. Klaus Fendler
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Bamberg, E., Clarke, R.J. & Fendler, K. Electrogenic Properties of the Na+,K+-ATPase Probed by Presteady State and Relaxation Studies. J Bioenerg Biomembr 33, 401–405 (2001). https://doi.org/10.1023/A:1010667407003

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  • DOI: https://doi.org/10.1023/A:1010667407003

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