Abstract
The humoral immunity, spleen and thymus weight indices, lymphocyte count in the thymus cortex, and granuloma diameter at vaccination sites were assessed in four differently immunopotentiated groups of meat chicken breeders. Breeders in the first two groups were given a killed Salmonella enterica serotype Enteritidis (SE) vaccine subcutaneously at 15 and 19 weeks of age. Breeders in the third and fourth groups were left unvaccinated. Breeders in the first group were further immunopotentiated with zinc and thymulin. Each bird in the first group was given the immunopotentiators intraperitoneally in a volume of 0.1 ml at intervals of 3 days for a period of 3 weeks, starting at 15 weeks of age. At each time, each bird in the first group received thymulin (10 ng) and ZnCl2 (1 μmol/L), using a carboxymethyl cellulose carrier, totalling 90 ng thymulin and 9 μmol of ZnCl2 per bird. Each bird in the first three groups was challenged orally with 6.7×106 cfu/ml of highly virulent SE organisms, at an age of 22 weeks. The first group, which had received zinc and thymulin, had the earliest and highest humoral immune response to SE (p<0.05). This was observed at 2 and 4 weeks after the first vaccination. In addition, the first group had the highest mean thymus weight index, and the highest mean lymphocyte count in the thymus cortex. No significant difference was observed between the first two vaccinated groups in the mean granuloma diameter developed at the two vaccination sites 48 h after administration of the vaccine (p>0.05).
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REFERENCES
American Association of Avian Pathologists, 1997. In: S.B. Hitcher, C.H. Domermuth, H.G. Purchase and J.E. Williams (eds), Isolation and Identification of Avian Pathogens, 4th edn, (College Station Press, College Station, TX)
Barbour, E.K. and Newman, J.A., 1989. Comparison of Mycoplasma gallisepticum subunit and whole organism vaccines containing different adjuvants by Western immunoblotting. Journal of Veterinary Immunology and Immunopathology, 22, 135-144
Barbour, E.K., Frerichs, W.M., Nabbut, N.H., Poss, P.E. and Brinton, M.K., 1993. Evaluation of bacterins containing three predominant phage types of Salmonella enteritidis for prevention of infection in egg-laying chickens. American Journal of Veterinary Research, 54, 1306-1309
Barbour, E.K., Hamadeh, S.K., Abi Ghanem, D., Haddad, J.J. and Safieh-Garabedian, B., 1998. Humoral and cell-mediated immunopotentiation in vaccinated chicken layers by thymic hormones and zinc. Vaccine, 16, 1650-1655
Barbour, E.K., Jurdi, L.H., Talhouk, R., Qatanani, M., Eid, A., Sakr, W., Bouljihad, M. and Spasojevic, R., 1999. Emergence of Salmonella Enteritidis outbreaks in broiler chickens of Lebanon: epidemiologic markers and competitive exclusion control. Scientific and Technical Review (World Animal Health Organization), 18, 710-718
Barbour, E.K., Jurdi, L.H., Faroon, O.M., Daghir, N.J. and Bouljihad, M., 2000. Chronological recognition by chicken of antigenic polypeptides in Salmonella Enteritidis with different plasmid profiles: relationship to infection rate. The Journal of Veterinary Medical Science, 62, 1139-1143
Barrow, P.A., Lovell, M.A. and Berchieri, A., 1991. The use of two live attenuated vaccines to immunize egg-laying hens against Salmonella enteritidis phage type 4. Avian Pathology, 20, 681-692
Buckingham, J.C., Safieh, B., Singh, S., Arduino, L.A., Cover, P.O. and Kendall, M.D., 1992. Interactions between the hypothalamo-pituitary adrenal axis and the thymus in the rat: a role of corticotrophin in the control of thymulin release. Journal of Neuroendocrinology, 4, 295-301
Campbell, T.W., 1986. In: G.J. Harrison and L.R. Harrison (eds), Clinical Avian Medicine and Surgery, (W.B. Saunders, Philadelphia), 250-273
Centers for Disease Control, 1992. Outbreaks of Salmonella enteritidis infection associated with consumption of raw shell eggs. Morbidity and Mortality Weekly Report, 41, 369-372
Centers for Disease Control, 1993. Outbreaks of Salmonella enteritidis gastroenteritis-California. Morbidity and Mortality Weekly Report, 42, 793-797
Chart, H., Threlfall, E.J. and Rowe, B., 1989. Virulence of Salmonella enteritidis phage type 4 is related to the possession of a 38 MDa plasmid. Federal European Microbiological Society — Microbiology Letters, 58, 299-304
Cowden, J.M., Lynch, D., Joseph, C.A., O'Mahony, M., Mawser, S.L., Spain, G.E., Ward, L. and Row, B., 1989. Case-control study of infections with Salmonella enteritidis phage type 4 in England. British Medical Journal, 299, 771-773
Dardenne, M., Boukaiba, N., Gagnerault, M.C., HomoDelarche, F., Chappuis, P., Lemonnier, D. and Savino, W., 1993. Restoration of the thymus in aging mice by in vivo zinc supplementation. Clinical Immunology and Immunopathology, 66, 127-135
Fields, P.I., Swanson, R.V., Haidaris, C.G. and Heffron, F., 1986. Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent. Proceedings of the National Academy of Science of the USA, 83, 5189-5193
Gast, R.K. and Beard, C.W., 1993. Research to understand and control Salmonella enteritidis in chickens and eggs. Poultry Science, 72, 1157-1163
Gast, R.K., Stone, H.D., Holt, P.S. and Beard, C.W., 1992. Evaluation of the efficacy of an oil-emulsion bacterin for protecting chickens against Salmonella enteritidis. Avian Diseases, 36, 992-999
Hassan, J.O. and Curtiss, R., 1994. Virulent Salmonella typhimurium-induced lymphocyte depletion and immunosuppression in chickens. Infection and Immunity, 62, 2027-2036
Incefy, G.S., Mertelsmann, R., Yata, K., Dardenne, J.M., Bach, J.F. and Gook, R.A., 1980. Induction of differentiation in human marrow T cell precursors by the synthetic thymic factor, FTS. Clinical and Experimental Immunology, 40, 396-406
Komuro, K. and Boyse, E., 1973. Induction of T lymphocytes from precursor cells in vitro by a product of the thymus. Journal of Experimental Medicine, 138, 479-482
Mish, B., Griffin, P.M., Tauxe, R.V., Cameron, D.N., Hutcheson, R.H. and Schaffner, W., 1991. Salmonella enteritidis gastroenteritis transmitted by intact chicken eggs. Annals of Internal Medicine, 115, 190-194
Murthy, K.K. and Regland, W.L., 1992. Effect of thymic extract on blastogenic responses of chickens. Poultry Science, 71, 311-315
National Research Council, 1984. Nutrient Requirements of Poultry, 8th edn, (National Academy Press, Washington, DC), 11-15
Nakamura, M., Nagamine, N., Takahashi, T., Suzuki, S. and Sato, S., 1994. Evaluation of the efficacy of a bacterin against Salmonella enteritidis infection and the effect of stress after vaccination. Avian Diseases, 38, 717-724
Okamoto, M., Morishita, M., Setoguchi, C. and Nakata, K., 1993. Restorative effect of short term administration of thymulin on thymus-dependent antibody production in restraint-stressed mice. International Journal of Immunology, 15, 757-762
Parent, G., Chevalier, P., Zalles, L., Sevilla, R., Bustos, M., Dhenin, J.M. and Jambon, B., 1994. In vitro lymphocyte-differentiating effects of thymulin (Zn-FTS) on lymphocyte subpopulations of severely malnourished children. American Journal of Clinical Nutrition, 60, 274-278
Perales, I. and Audicana, A., 1989. The role of hens' eggs in outbreaks of salmonellosis in north Spain. International Journal of Food Microbiology, 8, 175-180
Saha, A.R., Hadden, E.M. and Haden, J.W., 1995. Zinc induces thymulin secretion from human thymic epithelial cells in vitro and augments splenocyte and thymocyte responses in vivo. International Journal of Immunopharmacology, 17, 729-733
St. Louis, M.E., Morse, D.L., Potter, M.E., DeMelfi, T.M., Guzewich, J.J., Tauxe, R.V. and Blake, P.A., 1988. The emergence of grade A eggs as a major source of Salmonella enteritidis infections. New implications for the control of salmonellosis. Journal of the American Medical Association, 259, 2103-2107
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Barbour, E., Hamadeh, S., Bejjani, N. et al. Immunopotentiation of a Developed Salmonella enterica serotype Enteritidis Vaccine by Thymulin and Zinc in Meat Chicken Breeders. Vet Res Commun 25, 437–447 (2001). https://doi.org/10.1023/A:1010654818923
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DOI: https://doi.org/10.1023/A:1010654818923